Tissue Plasminogen Activator as an Antiangiogenic Agent in Experimental Laser-induced Choroidal Neovascular ization in Mice

Research Project

Project/Area Number	16K20323
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Ophthalmology
Research Institution	Nagoya City University
Principal Investigator	Mizutani Takeshi 名古屋市立大学, 大学院医学研究科, 助教 (70770428)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	脈絡膜新生血管 / プラスミノーゲン活性化因子 / CNV / ｔPA / 組織プラスミノーゲン活性化因子 / tPA / 加齢黄斑変性
Outline of Final Research Achievements	We investigate the antiangiogenic efficacy of tissue plasminogen activator (tPA) on experimental laser-induced choroidal neovascularization (CNV) in mice. Intravitreal administration of tPA significantly suppressed CNV leakage and CNV volume. Intravitreal injection of tPA suppressed fibrin/fibrinogen and CD31 expression in laser-induced lesions. Histologic examination and electroretinography showed no evidence of retinal toxicy in eyes injected with tPA. The current results suggested that tPA may be a potential therapeutic adjuvant for treating CNV.