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Functional analysis of inflammation-related miRNA in skin wound healing and homeostasis

Research Project

Project/Area Number 16K20361
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plastic surgery
Research InstitutionNagasaki University

Principal Investigator

TANAKA Katsuya  長崎大学, 医歯薬学総合研究科(医学系), 研究協力員 (70722750)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsmiRNA / 炎症 / イメージング / 皮膚創傷治癒 / 好中球 / 感染 / 黄色ブドウ球菌 / microRNA / 瘢痕・ケロイド / イメージング解析
Outline of Final Research Achievements

MicroRNAs are small noncoding RNAs that regulate protein translation by binding to complementary target mRNAs. In this study, we generated miR-142 knockout (KO) mice and showed that healing of Staphylococcus aureus-infected skin wounds was significantly delayed in miR-142 KO mice compared with that in wild-type mice. MiR-142 KO neutrophils showed altered phagocytosis as a consequence of chemotactic behavior, including enhanced F-actin assembly, disturbed cell polarity, and increased cell motility. We showed that these changes were linked to cytoskeletal regulation, and that expression of the small GTPases was markedly increased in miR-142-/- neutrophils. Collectively, our data demonstrate that the miR-142 family is indispensable for protection against S. aureus infection and its clearance at wound sites. MiR-142-3p and miR-142-5p play nonredundant roles in actin cytoskeleton regulation by controlling small GTPase translation in neutrophils at wound sites.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Book (1 results)

  • [Int'l Joint Research] ブリストル大学(英国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Total resection combined with osteotomy is more effective for orbital solitary fibrous tumor excision: a report of three cases2017

    • Author(s)
      Tanaka K, Yano H, Hayashi H, Hirano A
    • Journal Title

      International Ophthalmology

      Volume: 38 Issue: 1 Pages: 345-351

    • DOI

      10.1007/s10792-016-0421-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MiR-142 Is Required for Staphylococcus aureus Clearance at Skin Wound Sites via Small GTPase-Mediated Regulation of the Neutrophil Actin Cytoskeleton2017

    • Author(s)
      Tanaka K, Kim SE, Yano H, Matsumoto G, Ohuchida R, Ishikura Y, Araki M, Araki K, Park S, Komatsu T, Hayashi H, Ikematsu K, Tanaka K, Hirano A, Martin P, Shimokawa I, Mori R
    • Journal Title

      J Invest Dermatol

      Volume: 137 Issue: 4 Pages: 931-940

    • DOI

      10.1016/j.jid.2016.11.018

    • NAID

      40021436657

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] miR-142はsmall GTPaseを介して好中球の細胞骨格を調節し黄色ブドウ球菌の排除に関わっている2017

    • Author(s)
      田中 克弥
    • Organizer
      第26回日本形成外科学会基礎学術集会
    • Related Report
      2017 Annual Research Report
  • [Book] 臨床免疫・アレルギー科2018

    • Author(s)
      森 亮一、田中 克弥、野崎 中成、下川 功
    • Total Pages
      7
    • Publisher
      科学評論社
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2022-06-16  

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