the analysis of hair guiding ability depending on size of fibroblast clumps

• Project/Area Number: 16K20367
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: Nishida Michi 慶應義塾大学, 医学部(信濃町), 助教 (50748902)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 毛包誘導能 / 毛包誘導 / 凝集塊 / 線維芽細胞 / 毛 / 毛包 / 細胞凝集塊 / 未分化能

Outline of Final Research Achievements

In this study, in order to investigate the aggregation state of clumps on the premise of clinical application of hair induction by fibroblast lumps, the expression levels of undifferentiated markers based on the size of aggregate were compared and examined.
As a result of comparing the expression of various markers related to hair follicle induction using groups of three groups of different sizes, the expression of cxcr4 and prom1 (CD133) increased significantly as cell clumps increased. Currently, we are studying the conditions of aggregate formation effective for hair guidance for final implantation.