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Ex vivo angiogenic cell expansion system increases the number and vasculogenic potential of endothelial progenitor cells by switching the culture gravity condition

Research Project

Project/Area Number 16K20370
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plastic surgery
Research InstitutionJuntendo University

Principal Investigator

Hagiwara Hiroko  順天堂大学, 医学部, 博士研究員 (30589429)

Research Collaborator OGAWA Shiho  宇宙航空研究開発機構, 有人宇宙技術部門・きぼう利用センター・きぼう利用企画グループ, グループ長
TANAKA Rica  順天堂大学, 医学部, 先任准教授 (70509827)
KANAZAWA Shigeyuki  順天堂大学, 医学部, 非常勤講師 (50506243)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords血管再生 / 宇宙医学 / 血管内皮前駆細胞 / 創傷治癒 / 細胞移植 / 糖尿病性潰瘍 / 血管内皮前駆細胞 (EPC) / 細胞移植療法
Outline of Final Research Achievements

The CD34-positive cell number was significantly higher in the cultured with microgravity groups than in the normal control group. EPC number was significantly increased in the cultured with microgravity and earthgravity group compared to the control groups. dEPC-CFU were significantly increased in the cultured with both gravity group compared to the normal control. Furthermore, VEGF-A expression increased in the cultured with both gravity condition group compared to the normal control.In Conclusion, stimulation of MNC-QQc cells with MG increased the number of EPCs, such as CD34-positive cells, by enhancing their proliferation capacity. Furthermore, earth culture after microgravity stimulation induced vasculogenic differentiation of CD34 cells.
This study indicated that MNC-QQc in combination with Micro gravity-Earth gravity conditions might be a more effective angiogenic cell expansion culture method and could be a valuable tool for therapeutic vasculogenesis and tissue regeneration

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (9 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Evolution of autologous endothelial progenitor cell therapy for tissue regeneration and vasculogenesis.2016

    • Author(s)
      Hiroko Hagiwara, Tanaka Rica
    • Journal Title

      Personalized Medicine Universe

      Volume: 5 Pages: 8-15

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 重症下肢虚血に対する血管再生療法の現状2016

    • Author(s)
      萩原裕子、田中里佳
    • Journal Title

      日本下肢救済・足病学会誌

      Volume: 8 Pages: 123-129

    • NAID

      130005266742

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 微小重力環境が血管内皮前駆細胞に及ぼす影響と効果の検証2018

    • Author(s)
      萩原裕子,田中里佳,東端 晃,小川志保,金澤成行,水野博司
    • Organizer
      第17回 日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 血管内皮前駆細胞の培養効率化を目指した微小重力環境下生体外増幅培養法の確立2017

    • Author(s)
      萩原裕子、田中里佳、東端晃、小川 志保、金澤成行、水野 博司
    • Organizer
      第16回日本再生医療学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Quality and Quantity Control Cell Culture with Microgravity increases CD34-positive fraction and angiogenic potential of endothelial progenitor cells2017

    • Author(s)
      Hagiwara H, Higashibata A, Ogawa S, Kanazawa S, Mizuno H, Tanaka R
    • Organizer
      The 62nd Plastic surgery Research council
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Quality and Quantity Control Cell Culture with Microgravity to enhance vasculogeneis of peripheral blood endothelial progenitor cells2017

    • Author(s)
      Hagiwara H, Higashibata A, Ogawa S, Kanazawa S, Mizuno H, Tanaka R
    • Organizer
      Tissue Engineering Regenerative medicine international society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微小重力環境下における生体外増幅培養血管内皮細胞の機能解析2017

    • Author(s)
      萩原裕子,田中里佳,東端 晃,小川志保,金澤成行,水野博司
    • Organizer
      第26回日本形成外科学会基礎学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 血管内皮前駆細胞を含む末梢血単核球の微小重力環境下における培養法の確立2016

    • Author(s)
      萩原裕子、田中里佳、東端晃、小川 志保、金澤成行、水野 博司
    • Organizer
      第25回日本形成外科学会基礎学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞再生治療の奥義:実用化を見据えた技術の確立2016

    • Author(s)
      田中里佳、門真起子、有田佳代、藤村聡、萩原裕子、向後泰司、水野博司
    • Organizer
      第8回日本創傷外科学会総会・学術集会
    • Place of Presentation
      東京
    • Year and Date
      2016-07-21
    • Related Report
      2016 Research-status Report
  • [Presentation] Challenges of stem cell therapy clinical trial for non-healing extremity wounds. Efficiancy and safety evaluation of QQc cultured ex vivo expanded peripheral blood  stem cell therapy prior to clinical translation2016

    • Author(s)
      Rica Tanaka, Makiko Kado, Satoshi Fujimura, Kayo Arita, Kayoko Tono-Okada, Hiroko Hagiwara, Hiroshi Mizuno
    • Organizer
      The 13th Japan-Korea Congress of  Plastic and Reconstructive Surgery
    • Place of Presentation
      金沢
    • Year and Date
      2016-05-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 再生新法下における難治性潰瘍に対する次世代血管再生治療の研究再開までの道のりと今後の展望2016

    • Author(s)
      田中里佳、藤村聡、有田佳代、門真起子、市川 佑一、萩原裕子、水野博司
    • Organizer
      第59回日本形成外科学総会・学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-04-13
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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