Development of approaches to control vascular permeability using mouse models with increased vascular permeability

• Project/Area Number: 16K20379
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Chiba University
• Principal Investigator: SHIMAZUI Takashi 千葉大学, 医学部附属病院, 医員 (20598239)
• Research Collaborator: NAKADA Taka-aki 千葉大学, 大学院医学研究院, 講師 ; HATANO Masahiko 千葉大学, 大学院医学研究院, 教授 ; FUJIMURA Lisa 千葉大学, バイオメディカル研究センター, 助教
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 動物実験 / 血管透過性亢進 / in vivo imaging / 敗血症

Outline of Final Research Achievements

Animal study is helpful to investigate therapeutic approaches to control increased vascular permeability, which worsens clinical outcomes in critically ill patients. However, conventional technique to quantify vascular permeability requires sacrifice of animals. We aimed to develop a non-invasive technique to quantify vascular permeability in mouse models, and then, investigated new therapeutic approaches to control vascular permeability. First, we found footpad fluorescent intensity assessed by an in vivo fluorescent imaging system had a strong correlation to lung fluorescent intensity, and had a same trend of severity of lung vascular permeability assessed by a conventional technique. Thus, the footpad fluorescent intensity assessment can be use to a non-invasive technique to quantify lung vascular permeability. Next, we found the erythropoietin may control vascular permeability in septic mouse. Further studies are warranted to establish ways to control vascular permeability.

Research Products

• [Journal Article] Development of Noninvasive In Vivo Approach to Assess Vascular Permeability in Inflammation Using Fluorescence Imaging. (2017)
  • Author(s): Shimazui T, Nakada TA, Fujimura L, Sakamoto A, Hatano M, Oda S.
  • Journal Title: Shock. Volume: - Issue: 6 Pages: 1-1
  • DOI: 10.1097/shk.0000000000001075
  • Peer Reviewed / Open Access
• [Presentation] Noninvasive technique using fluorescence imaging to assess vascular permeability in sepsis (2018)
  • Author(s): Shimazui T, Nakada TA, Fujimura L, Sakamoto A, Hatano M, Oda S
  • Organizer: International Symposium on Intensive Care and Emergency Medicine
  • Int'l Joint Research