Analysis of novel transcription factors controlling cartilage formation

Research Project

Project/Area Number	16K20420
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Functional basic dentistry
Research Institution	Osaka University
Principal Investigator	Yoshifumi Takahata 大阪大学, 歯学研究科, 助教 (60635845)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	転写因子 / 軟骨細胞 / シグナル伝達 / Sox9 / 細胞・組織 / 歯学 / 生理活性 / 薬理学
Outline of Final Research Achievements	Transcription factors belonging to completely different families are responsible for cell differentiation from undifferentiated mesenchymal stem cells to various skeletal cells. Transcription factor Sox9 is essential as a master regulator of chondrocyte differentiation, however the upstream transcription factors regulating this Sox9 are still unknown. In this study, a novel upstream transcription factor (Inducer of transcription factor Sox 9: Itfs) is identified that controls the Sox9 gene using the enChIP method applying genomic editing technology. Further analysis of the intracellular signaling of the transcription factor revealed that the Itfs protein is stabilized by the treatment of BMP2.

Report

(3 results)