Glucagon-GLP-1 conversion by osteocalcin in pancreatic alpha cells
| Project/Area Number |
16K20421
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オステオカルシン / GLP-1 / GLP-1受容体欠損マウス / グルカゴン / 糖代謝 / インクレチン / オステカルシン / α細胞 |
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| Outline of Final Research Achievements |
Stimulation of isolated pancreatic islet with osteocalcin (OC) increased the expression of PC1/3, a processing enzyme that liberates GLP-1 from proglucagon. Moreover, GLP-1 positive cells were significantly increased in the pancreatic sections from mice treated with OC for 4 weeks. However, these phenotypes were not observed when GLP-1 receptor knockout mice (GLP-1R KO) were used. These results indicate that OC promotes GLP-1 production from alpha cells, and GLP-1 receptor signaling is required in the process.
While long-term oral administration of OC improved glucose tolerance in female wild-type mice, it rather triggered glucose intolerance and adipocyte hypertrophy in GLP-1R KO mice. Thus, it is suggested that GLP-1 receptor signaling is required for an improvement of glucose tolerance by OC.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Differential roles of carboxylated and uncarboxylated osteocalcin in prostate cancer growth2016
Author(s)
Hayashi, Y., Kawakubo-Yasukochi, T., Mizokami, A., Takeuchi, H., Nakamura, S. and Hirata, M.
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Journal Title
J. Cancer
Volume: 7
Issue: 12
Pages: 1605-1609
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Differential role of SNAP-25 phosphorylation by protein kinases A and C in the regulation of SNARE complex formation and exocytosis in PC12 cells2016
Author(s)
Gao, J., Hirata, Makiko, Mizokami, A., Zhao, J., Takahashi, I., Takeuchi, H. and Hirata, Masato
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Journal Title
Cell. Signal.
Volume: 28
Issue: 5
Pages: 425-437
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration2016
Author(s)
Mizokami, A., Wang, DG, Tanaka, M., Gao, J., Takeuchi, H., Matsui, T. and Hirata, M.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 80
Issue: 11
Pages: 2176-2183
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Uncarboxylated osteocalcin increases serum nitric oxide levels and ameliorates hypercholesterolemia in mice fed an atherogenic diet.2016
Author(s)
Kondo, A., Kawakubo-Yasukochi, T., Mizokami, A., Chishaki, S., Takeuchi, H. and Hirata, M.
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Journal Title
eJBio
Volume: 13
Pages: 22-28
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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