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The role of PPP1r18, an actin binding protein, in osteoclastic bone resorption

Research Project

Project/Area Number 16K20423
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

Matsubara Takuma  九州歯科大学, 歯学部, 助教 (00423137)

Co-Investigator(Renkei-kenkyūsha) JIMI Eijiro  九州歯科大学, 分子情報生化学分野, 教授 (40276598)
KOKABU Shoichiro  九州歯科大学, 分子情報生化学分野, 准教授 (30448899)
Research Collaborator NAKATOMI Chihiro  
URATA MARIKO  
TOYAMA Kenya  
KOBAYAKAWA Miki  
YAGINUMA Tatsuki  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords破骨細胞 / 骨吸収 / Src / 骨代謝 / 細胞骨格
Outline of Final Research Achievements

Osteoclasts in tyrosine kinase Src deficient mice that show osteopetrosis hardly resorb bone matrix because of disable to attachment to bone matrix. Src organizes actin accumulation and regulates actin ring formation for attachment. However molecular mechanisms how Src regulates actin ring formation in osteoclasts are not fully understood. We identified an actin binding protein PPP1r18 as Src binding protein by mass spectrum analysis. PPP1r18 was expressed and localized in actin ring with Src of osteoclasts. These results suggest that PPP1r18 bind to Src and involved in actin ring formation. Downregulation of PPP1r18 expression promoted actin ring formation. On the other hand, overexpression of PPP1r18 inhibited actin ring formation and bone resorption. Mutation of protein phosphatase 1 binding domain of PPP1r18 canceled inhibition of actin ring formation by PPP1r18. These results suggest that PPP1r18 negatively regulates actin ring formation and bone resorption.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (33 results)

All 2018 2017 2016

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (29 results) (of which Int'l Joint Research: 11 results)

  • [Journal Article] The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.2018

    • Author(s)
      Kokabu S, Nakatomi C, Matsubara T, Ono Y, Addison WN, Lowery JW, Urata M, Hudnall AM, Hitomi S, Nakatomi M, Sato T, Osawa K, Yoda T, Rosen V, Jimi E.
    • Journal Title

      Journal of Biological Chemistry.

      Volume: 292 Issue: 31 Pages: 12885-12894

    • DOI

      10.1074/jbc.m116.774570

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Actin-Binding Protein PPP1r18 Regulates Maturation, Actin Organization, and Bone Resorption Activity of Osteoclasts2018

    • Author(s)
      Takuma Matsubara、Shoichiro Kokabu、Chihiro Nakatomi、Masayuki Kinbara、Toshihiro Maeda、Mitsuhiro Yoshizawa、Hisataka Yasuda、Teruko Takano-Yamamoto、Roland Baron、Eijiro Jimi
    • Journal Title

      Molecular and cellular biology

      Volume: 38 Issue: 4

    • DOI

      10.1128/mcb.00425-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Differentiation and Actin Ring Formation in Osteoclasts2018

    • Author(s)
      Matsubara Takuma、Kinbara Masayuki、Maeda Toshihiro、Yoshizawa Mitsuhiro、Kokabu Shoichiro、Takano Yamamoto Teruko
    • Journal Title

      Mathews Journal of Cytology and Histology

      Volume: 1 Pages: 1-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of osteoclast differentiation and actin ring formation by the cytolinker protein plectin.2017

    • Author(s)
      Matsubara T, Kinbara M, Maeda T, Yoshizawa M, Kokabu S, Takano Yamamoto T.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: Aug 5;489(4) Issue: 4 Pages: 472-476

    • DOI

      10.1016/j.bbrc.2017.05.174

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A novel c-Src binding protein PPP1r18 regulates actin ring in osteoclasts2017

    • Author(s)
      Takuma Matsubara, Chihiro Nakatomi, Shoichiro Kokabu, Eijiro Jimi
    • Organizer
      Asia-Pacific Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of the binding region of NF-κB-p65 on Smad42017

    • Author(s)
      Mariko Urata, Takuma Matsubara, Shoichiro Kokabu, Chihiro Nakatomi, Min Zhang, Shizu Hirata-Tsuchiya, Chiaki Kitamura and Eijiro Jimi
    • Organizer
      Asia-Pacific Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of BMP signaling in EMT of malignant melanoma2017

    • Author(s)
      Masahiro Ogawa, Shoichiro Kokabu,Chihiro Nakatomi, Takuma Matsubara, Seiji Watanabe and Eijiro Jimi
    • Organizer
      Asia-Pacific Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NF-κB regulates the differentiation of skeletal muscle stem cells2017

    • Author(s)
      Yuri Nakaminami, Yu Akaike, Katsuya Shirai, Shoichiro Kokabu, Takuma Matsubara and Eijiro Jimi
    • Organizer
      Asia-Pacific Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] TLE3 regulates myogenic differentiation of muscle stem cells by repressing the transcriptional activity of MyoD2017

    • Author(s)
      Shoichiro Kokabu, Chihiro Nakatomi, Takuma Matsubara and Eijiro Jimi
    • Organizer
      Asia-Pacific Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A p130Cas binding protein Bif-1 is involved in osteoclastic bone resorption2017

    • Author(s)
      Kenya Touyama, Takuma Matsubara, Shoichiro Kokabu, Chihiro Nakatomi, Kenshi Maki and Eijiro Jimi
    • Organizer
      Asia-Pacific Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An actin binding protein PPP1r18 regulates actin ring in osteoclasts through Protein phosphatase binding2017

    • Author(s)
      Takuma Matsubara, Chihiro Nakatomi, Shoichiro Kokabu, Eijiro Jimi
    • Organizer
      The 65th annual meeting of Japanese association for dental research
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression levels of umami receptor, tas1r1/tas1r3, are decreased in an in vitro model of skeletal muscle atrophy2017

    • Author(s)
      Miki Kobayakawa, Tsuyoshi Nakajima, Takuma Matsubara, William Addison, Ryuji Hosokawa, Shoichiro Kokabu
    • Organizer
      The 10th Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] p130Cas is involved in morphological change, mobility, and invasion ability in OSCC2017

    • Author(s)
      Tatsuki Yaginuma, Takuma Matsubara, Shoichiro Kokabu,Chihiro Nakatomi, Izumi Yosioka, Eijiro Jimi
    • Organizer
      The 10th Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A p130Cas binding protein Bif-1 is involved in osteoclastic bone resorption.2017

    • Author(s)
      Kenya Touyama, Takuma Matsubara, Shoichiro Kokabu, Chihiro Nakatomi, Kenshi Maki and Eijiro Jimi
    • Organizer
      The 10th Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規c-Src会合分子PPP1r18は破骨細胞の骨吸収を負に制御する2017

    • Author(s)
      松原琢磨、古株彰一郎、中富千尋、自見英治郎
    • Organizer
      第77回九州歯科学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 悪性黒色腫の上皮間葉移行におけるBMPシグナルの役割2017

    • Author(s)
      小川昌洋、古株彰一郎、中富千尋、松原琢磨、渡邉誠之、自見英治郎
    • Organizer
      第77回九州歯科学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] p130Casと結合するタンパク質Bif-1は骨吸収に関与する2017

    • Author(s)
      當山健弥、松原琢磨、古株彰一郎、中富千尋、牧 憲司、自見英治郎
    • Organizer
      第77回九州歯科学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Smad4とNF-κB p65の結合領域の検討2017

    • Author(s)
      浦田真梨子、松原琢磨、古株彰一郎、中富千尋、土屋-平田志津、鷲尾絢子、諸冨孝彦、北村知昭、自見英治郎
    • Organizer
      第77回九州歯科学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TLE3は骨格筋組織幹細胞 サテライト細胞の筋分化を制御する2017

    • Author(s)
      古株彰一郎、中富千尋、松原琢磨、自見英治郎
    • Organizer
      第77回九州歯科学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アクチン結合分子PPP1r18は破骨細胞のアクチンリング形成及び破骨細胞性骨吸収を制御する2017

    • Author(s)
      松原琢磨、古株彰一郎、中富千尋、自見英治郎
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] NF-kB非古典的経路の内軟骨内骨化過程における役割2017

    • Author(s)
      中富千尋、古株彰一郎、松原琢磨、自見英治郎
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TLE3はサテライト細胞の筋分化を制御する2017

    • Author(s)
      古株彰一郎、中富千尋、松原琢磨、自見英治郎
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 破骨細胞のアクチンリング形成および破骨細胞性骨吸収を制御するアクチン結合分子PPP1r182017

    • Author(s)
      松原琢磨、古株彰一郎、中富千尋、自見英治郎
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 成長板軟骨細胞の増殖におけるNF-κB非古典的経路の役割2017

    • Author(s)
      中富千尋,松原琢磨,中富満城,古株彰一郎,自見英治郎
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 悪性黒色腫の顎骨浸潤と転移におけるBMPシグナルの役割2017

    • Author(s)
      小川昌洋、古株彰一郎、中富千尋、松原琢磨、渡邉誠之、自見英治郎
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TLE3による骨格筋組織幹細胞サテライト細胞の増殖・分化制御機構2017

    • Author(s)
      古株彰一郎、中富千尋、松原琢磨、自見英治郎
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] BMP による骨形成促進を目的としたSmad4とNF-κB p65の会合領域の検討2017

    • Author(s)
      浦田真梨子、松原琢磨、古株彰一郎、中富千尋、土屋-平田志津、鷲尾絢子、諸冨孝彦、北村知昭、自見英治郎
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 破骨細胞アクチンリング形成を制御する分子PPP1r18の同定2016

    • Author(s)
      松原琢磨
    • Organizer
      Skeletal Science Retreat
    • Place of Presentation
      熱海
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規アクチン結合分子PPP1r18は破骨細胞のアクチンリング形成を阻害する2016

    • Author(s)
      松原琢磨 ,中富千尋 ,古株彰一郎 ,山本照子 ,自見英治郎
    • Organizer
      日本歯科基礎医学会
    • Place of Presentation
      北海道
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 破骨細胞のアクチンリング形成を制御する新規アクチン結合分子PPP1r182016

    • Author(s)
      松原琢磨 ,中富千尋 ,古株彰一郎 ,山本照子 ,自見英治郎
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規アクチン結合分子PPP1r18による破骨細胞のアクチンリング形成制御2016

    • Author(s)
      松原琢磨 ,中富千尋 ,古株彰一郎 ,自見英治郎
    • Organizer
      日本骨免疫学会,
    • Place of Presentation
      沖縄
    • Year and Date
      2016-07-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規アクチン結合分子PPP1r18による破骨細胞のアクチン細胞骨格制御2016

    • Author(s)
      松原琢磨 ,中富千尋 ,古株彰一郎 ,浦田真梨子 ,自見英治郎
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Year and Date
      2016-05-28
    • Related Report
      2016 Research-status Report
  • [Presentation] A novel protein actin ring regulator PPP1r18 in osteoclasts2016

    • Author(s)
      Takuma Matsubara, Chihiro Nakatomi, Mariko Urata, Masahiro Ogawa, Kenya Toyama, Hiroshi Kunihiro, Shoichiro Kokabu and Eijiro Jimi
    • Organizer
      Asia-pacific conference in Fukuoka 2016
    • Place of Presentation
      Kitakyushu
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-12-27  

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