| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
S. sanguinis had the activity to induce secretion of IL-1α by a murine dendritic cell (XS-106) and a murine bone marrow-derived macrophage (BMMs) from C57BL/6 in a dose-dependent manner, but the activity toward NLRP3-, ASC- and caspase-1-deficient BMMs were significantly attenuated. The calpain-1 inhibitor MDL28170 but not the caspase 1 inhibitor z-YVAD-fmk attenuated the IL-1α-inducing activity. Cytochalasin D, an inhibitor of actin polymerization reduced the IL-1α-inducing activity. The receptor P2X/P2Y inhibitor oxidized ATP downregulated IL-1α release. Colocalization of caspase-1 with IL-1α was detected by Proximity ligation assay.
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