Effects of polyphenols on doxorubicin-induced oral keratinocyte cytotoxicity and anticancer potency against oral cancer cells.

• Project/Area Number: 16K20441
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tohoku University
• Principal Investigator: SHENG HONG 東北大学, 歯学研究科, 大学院非常勤講師 (60771904)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ドキソルビシン / ヒト口腔正常ケラチノサイト / レスベラトロール / 没食子酸エピガロカテキン / タンニン酸 / 細胞傷害 / ポリフェノール / 細胞損傷 / 細胞死 / 抗酸化作用 / 口腔扁平上皮癌細胞 / 抗ガン剤 / 作用機序

Outline of Final Research Achievements

Normal human oral keratinocytes are highly sensitive to anticancer drugs including doxorubicin. Resveratrol, epigallocatechin gallate, and tannic acid are polyphenolic compounds that were reported to have cardioprotective effect when combined with doxorubicin. However, it is unknown whether these polyphenols could protect normal human oral keratinocytes against doxorubicin-induced cytotoxicity without weakening its cytotoxic potential against oral cancer cells. Here, we examined the effects of the 3 polyphenolic compounds on doxorubicin-induced cytotoxicity in normal human oral keratinocytes and also investigated their effects on doxorubicin potency in HSC-2 human oral squamous cell carcinoma cells.
This study for the first time reported the effects of resveratrol, epigallocatechin gallate, and tannic acid on doxorubicin-induced cytotoxicity in normal oral keratinocytes and oral cancer cells.

Research Products

• [Journal Article] Effects of polyphenols on doxorubicin-induced oral keratinocyte cytotoxicity and anticancer potency against oral cancer cells (2018)
  • Author(s): Sheng H, Ogawa T，Niwano Y，Sasaki K，Tachibana K
  • Journal Title: J Oral Pathol Med Volume: 印刷中 Issue: 4 Pages: 368-374
  • DOI: 10.1111/jop.12685
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Enhanced cell killing and apoptosis of oral squamous cell carcinoma cells with ultrasound in combination with cetuximab coated albumin microbubbles Journal of drug targeting. (2018)
  • Author(s): Narihira K, Watanabe A, Sheng H, Endo H, Feril LB, Irie Y, Ogawa K, Moosavi-Nejad S, Kondo S, Kikuta T, Tachibana K.
  • Journal Title: J Drug Target. Volume: 26 Issue: 3 Pages: 278-288
  • DOI: 10.1080/1061186x.2017.1367005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prominent Anti-UV Activity and Possible Cosmetic Potential of Lignin-carbohydrate Complex. (2016)
  • Author(s): Sakagami H, Sheng H, Okudaira N, Yasui T, Wakabayashi H, Jia J, Natori T, Suguro-Kitajima M, Oizumi H, Oizumi T
  • Journal Title: In Vivo Volume: 30 Pages: 331-339
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Microbicidal action of photoirradiated aqueous extracts from wine lees (2016)
  • Author(s): Tsukada, M., Sheng, H., Kamachi, T. & Niwano, Y.
  • Journal Title: Journal of Food Science and Technology Volume: 1 Issue: 7 Pages: 1-8
  • DOI: 10.1007/s13197-016-2273-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Effects of polyphenols on doxorubicin-induced oral keratinocyte cytotoxicity and anticancer potency against oral cancer cells (2017)
  • Author(s): Sheng H, Ogawa T, Niwano Y, Sasaki K, Tachibana K.
  • Organizer: 14th meeting of thenternational society for maxillofacial rehabilitation-64th annual meeting of the american academy of maxillofacial prosthetics.
  • Int'l Joint Research
• [Presentation] Effects of polyphenols on doxorubicin-induced cytotoxicity in normal human oral keratinocytes (2017)
  • Author(s): Sheng H, Ogawa T, Niwano Y, Sasaki K, Tachibana K.
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] 超音波照射によるドキソルビシンおよびナノバブルを併用した抗癌効果 (2017)
  • Author(s): 生宏、渡邉晶子、成平恭一、立花克郎
  • Organizer: 日本超音波医学会第90回学術集会
• [Book] Nanostructures for Oral Medicine:Therapeutic potential of solubilized nanolignin against oral diseases (2017)
  • Author(s): Sakagami H, Sheng H, Yasui T, Fukuchi K, Oizumi T, Ohno H, Yamamoto M, Fukuda T, Kotohda K, Yoshida H, Kanamoto T, Terakubo S, Nakashima H
  • Publisher: Elsevier