Analysis of promoting in vitro multispecies biofilm formation by stress response with antimicrobial component

• Project/Area Number: 16K20451
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Niigata University
• Principal Investigator: Ohsumi Tatsuya 新潟大学, 医歯学系, 助教 (30759725)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: バイオフィルム / ストレス応答 / Calgary Biofilm Device / 共焦点レーザー顕微鏡 / 複合バイオフィルムモデル / 化学的制御 / バイオフィルム形成関連遺伝子 / sub-MIC / CLSM

Outline of Final Research Achievements

In this study, we investigated the mechanism of biofilm formation promotion by stress response under the action of low concentration antimicrobial component using in vitro complex biofilm model. After the action of chlorhexidine gluconate (CHG) below the minimum inhibitory concentration (sub-MIC), an increase in the amount of biofilm was observed but no increase in viable count was observed, so that the amount of matrix increased It was inferred. It was suggested that CHG of sub-MIC promotes biofilm formation by influencing the production of extracellular matrix, not bacterial growth in biofilm.

Research Products

• [Journal Article] The effect of chlorhexidine on dental calculus formation: an in vitro study (2018)
  • Author(s): Sakaue Yuuki、Takenaka Shoji、Ohsumi Tatsuya、Domon Hisanori、Terao Yutaka、Noiri Yuichiro
  • Journal Title: BMC Oral Health Volume: 18 Issue: 1
  • DOI: 10.1186/s12903-018-0517-3
  • Peer Reviewed / Open Access
• [Journal Article] 結核菌表層糖脂質誘導体の口腔バイオフィルム形成に与える影響 (2018)
  • Author(s): 長谷川泰輔, 竹中彰治, 小田真隆, 鈴木裕希, 坂上雄樹, 大墨竜也, 野杁由一郎
  • Journal Title: Bacterial Adherence & Biofilm Volume: 印刷中
• [Journal Article] Antimicrobial activity of ethylene-vinyl acetate containing bioactive filler against oral bacteria (2017)
  • Author(s): Nagai, K., Domon, H., Oda, M., Shirai, T., Ohsumi, T., Terao, Y., Arai, Y
  • Journal Title: Dental Materials Journal Volume: 36 Issue: 6 Pages: 784-790
  • DOI: 10.4012/dmj.2016-321
  • NAID: 130006224952
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access
• [Journal Article] Vizantin inhibits bacterial adhesion without affecting bacterial growth and causes Streptococcus mutans biofilm to detach by altering its internal architecture (2016)
  • Author(s): Takenaka S, Oda Masataka, Domon H, Suzuki Y, Ohshima H, Yamamoto H, Tarao Y, Noiri Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 480 Issue: 2 Pages: 173-179
  • DOI: 10.1016/j.bbrc.2016.10.021
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Sulfated vizantin inhibits bacterial adhesion without affecting bacterial growth and causes ex vivo oral biofilm to detach by hydrophilic surface property (2017)
  • Author(s): Hasegawa T, Takenaka S, Oda M, Suzuki Y, Sakaue Y, Ohsumi T, Noiri Y
  • Organizer: Niigata-Taiwan University Dental Research Symposium
  • Int'l Joint Research
• [Presentation] 結核菌表層糖脂質誘導体の口腔バイオフィルム形成に与える影響 (2017)
  • Author(s): 長谷川泰輔、竹中彰治、小田真隆、鈴木裕希、坂上雄樹、大墨竜也、野杁由一郎
  • Organizer: 第31回日本バイオフィルム学会学術集会
• [Presentation] Ex vivoモデルを用いた結核菌表層糖脂質誘導体（ビザンチン）のバイオフィルム形成への影響 (2017)
  • Author(s): 長谷川泰輔、竹中彰治、小田真隆、鈴木裕希、坂上雄樹、大墨竜也、野杁由一郎
  • Organizer: 第146回日本歯科保存学会2017年春季学術大会
• [Presentation] Sub-MICのグルコン酸クロルヘキシジンがin vitro複合バイオフィルム形成に与える影響 (2017)
  • Author(s): 鈴木裕希，大墨竜也，長谷川泰輔，竹中彰治, 野杁由一郎
  • Organizer: 第147回日本歯科保存学会2017年秋季学術大会
• [Presentation] in vitroバイオフィルムモデルを用いたHelicobacter pyloriの形態学的検索 (2017)
  • Author(s): 永田量子，大墨竜也，鈴木裕希，長谷川泰輔，竹中彰治, 野杁由一郎
  • Organizer: 第147回日本歯科保存学会2017年秋季学術大会
• [Presentation] 結核菌表層糖脂質誘導体のStreptococcus mutansバイオフィルム形成に与える影響 (2016)
  • Author(s): 竹中彰治、小田真隆、黒澤美絵、土門久哲、大墨竜也、寺尾豊、野杁由一郎
  • Organizer: 日本歯科保存学会2016年秋季学術大会（第145回）
  • Place of Presentation: キッセイ文化ホール（長野県松本市）
  • Year and Date: 2016-10-27
• [Book] Microbial Biofilms-Importance and Applications (2016)
  • Author(s): Takenaka, S., Oda, M., Domon, H., Wakamatsu, R., Ohsumi, T., Terao, Y., Noiri, Y.
  • Total Pages: 509
  • Publisher: InTech