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Establishment of periodontal ligament stem cells derived human induced pluripotent stem cells and effective regeneration of periodontal ligament tissues using these cells.

Research Project

Project/Area Number 16K20457
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionKyushu University

Principal Investigator

Hamano Sayuri  九州大学, 歯学研究院, 助教 (40757978)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsiPS細胞 / 歯根膜幹細胞 / 細胞外マトリックス / 神経堤細胞 / 細胞外基質
Outline of Final Research Achievements

In this study, we aimed to induce the differentiation of induced pluripotent stem (iPS) cells into periodontal ligament stem cells (PDLSCs) and evaluate their regenerative potential for periodontal ligament (PDL) tissues. After the culture of neural crest-like cells derived from iPS (iPS-NC) cells on the extracellular matrix (ECM) from human PDL cells, they showed the up-regulation of the expression of PDL and mesenchymal stem cell markers. When iPS-NC cells exposed to PDL cells-derived ECM were transplanted into rat PDL defects, they promoted the formation of new PDL- and alveolar bone-like tissues. These results suggested our culture method was crucially efficient to generate PDLSCs-like cells from iPS cells and these cells had the potential to induce PDL tissue regeneration.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2018 2017 2016

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells2018

    • Author(s)
      Hamano Sayuri、Tomokiyo Atsushi、Hasegawa Daigaku、Yoshida Shinichiro、Sugii Hideki、Mitarai Hiromi、Fujino Shoko、Wada Naohisa、Maeda Hidefumi
    • Journal Title

      Stem Cells and Development

      Volume: 27 Issue: 2 Pages: 100-111

    • DOI

      10.1089/scd.2017.0077

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glial cell line-derived neurotrophic factor from human periodontal ligament cells treated with proinflammatory cytokines promotes neurocytic differentiation of PC12 cells.2017

    • Author(s)
      Yoshida S, Yamamoto N, Wada N, Tomokiyo A, Hasegawa D, Hamano S, Mitarai H, Monnouchi S, Yuda A, Maeda H.
    • Journal Title

      J Cell Biochem.

      Volume: 118 Issue: 4 Pages: 699-708

    • DOI

      10.1002/jcb.25662

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells.2017

    • Author(s)
      芹田俊、友清淳、長谷川大学、濱野さゆり、杉井英樹、吉田晋一郎、水町博之、御手洗裕美、門野内聡、和田尚久、前田英史
    • Journal Title

      Archives of oral biology

      Volume: 78 Pages: 135-143

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells.2016

    • Author(s)
      Yoshida S, Wada N, Hasegawa D, Miyaji H, Mitarai H, Tomokiyo A, Hamano S, Maeda H.
    • Journal Title

      Journal of Dental Research

      Volume: 95 Issue: 11 Pages: 1282-1290

    • DOI

      10.1177/0022034516653085

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] iPS細胞由来の歯根膜幹細胞様細胞の樹立2018

    • Author(s)
      濱野さゆり、友清淳、長谷川大学、杉井英樹、吉田晋一郎、御手洗裕美、有馬麻衣、野津葵、藤野翔香、和田尚久、前田英史
    • Organizer
      第17回再生医療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] iPS細胞由来の歯根膜幹細胞様細胞の樹立2018

    • Author(s)
      濱野 さゆり
    • Organizer
      OBT研究センター キックオフシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Directing human iPS cells toward PDL stem cells2016

    • Author(s)
      濱野さゆり、友清淳、和田尚久、長谷川大学、杉井英樹、吉田晋一郎、芹田俊,、水町博之、御手洗裕美、前田英史
    • Organizer
      IADR
    • Place of Presentation
      CoEx Convention & Exhibition Center
    • Year and Date
      2016-06-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] iPS細胞由来の歯根膜幹細胞様細胞の樹立2016

    • Author(s)
      濱野さゆり、友清淳、和田尚久、長谷川大学、杉井英樹、吉田晋一郎、芹田俊,、水町博之、御手洗裕美、前田英史
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      栃木県総合文化センター
    • Year and Date
      2016-06-09
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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