Mechanisms of reparative dentin formation focusing on glycome-shift in human dental pulp cells
| Project/Area Number |
16K20469
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯髄 / 歯髄・象牙質複合体 / BMP-1 / 糖鎖 / グライコーム / 修復象牙質 / 歯学 / 細胞・組織 |
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| Outline of Final Research Achievements |
BMP-1 has been well investigated as a catalytic enzyme of procollagen I-III and dentin-specific non-collagenous proteins; however, how BMP-1 affects cell surface molecules remains unclear. In this study, we focused on changes in glycan structure, ie, glycome-shift by BMP-1, which was clarified from a comprehensive analysis by lectin microarray in human dental pulp cells. We found that the N-acetylgalactosamine modification was slightly increased, while the α2,6-sialic acid modification was decreased in insoluble fractions from human dental pulp cells. These results suggest that BMP-1 is involved not only in the maturation of collagen and dentin-specific proteins as protease but also in glyco-alteration of membrane proteins in human dental pulp cells.
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Report
(3 results)
Research Products
(7 results)
