• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establisment of regenerative techique for jaw bone using MSCs spheroids cultureing with floating condition.

Research Project

Project/Area Number 16K20480
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Prosthodontics/ Dental materials science and
Research InstitutionTohoku University

Principal Investigator

Niibe Kunimichi  東北大学, 大学病院, 助教 (50468500)

Research Collaborator EGUSA Hiroshi  
MATSUZAKI Yumi  
Zhang Maolin  
OHORI Yumi  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords間葉系幹細胞 / 神経堤幹細胞 / 振盪培養 / 浮遊培養 / 未分化性維持 / 骨再生 / スフェア / 未分化維持 / 再生医療 / 3次元培養 / 顎骨再生
Outline of Final Research Achievements

We successfully established the newly culture method for mesenchymal stem cells (MSCs) to keep their stem-ness under shaking condition. MSCs are getting spheroids with shaking culture method. MSC spheroids can supply undifferentiated MSCs for a long period. This technology is patent pending.
Moreover, We try to generate bone tissues with MSC spheroids to apply jaw bone regeneration. From our results, MSC spheroid can re-attach on a culture dish. Then, migrated cells from MSC spheroids are positive for alkaline phosphatase (ALP) after osteogenic induction. However, there are few positive cells in spheroids itself after ALP staining. It suggested that MSC spheroid kept their stem-ness robustly.
From these findings, we are trying to establish the newly osteogenic induction method using MSC spheroids.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Mayo clinic(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Bone marrow PDGFRa+Sca-1+ enriched mesenchymal stem cells support survival of and antibody production by plasma cells in vitro through IL-6.2018

    • Author(s)
      Atsuko Kayaba, Ari Itoh-Nakadai, Kunimichi Niibe, Matsuyuki Shirota, Ryo Funayama, Akiko Sugahara-Tobinai, Yi Li Wong, Masanori Inui, Keiko Nakayama, and Toshiyuki Takai.
    • Journal Title

      Int Immu

      Volume: in press Issue: 6 Pages: 241-253

    • DOI

      10.1093/intimm/dxy018

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Challenges for stem cell-based “regenerative prosthodontics”2017

    • Author(s)
      Niibe K, Suehiro F, Oshima M, Nishimura M, Kuboki T, Egusa H
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 61 Issue: 1 Pages: 3-5

    • DOI

      10.1016/j.jpor.2016.09.001

    • NAID

      130005465202

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry.2017

    • Author(s)
      Niibe K, Zhang M, Nakazawa K, Morikawa S, Nakagawa T, Matsuzaki Y, Egusa H.
    • Journal Title

      Japanese Dental Science Review

      Volume: 印刷中

    • NAID

      50012041277

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 神経堤の特徴を呈する間葉系幹細胞塊を用いた再生歯科医療技術の開発2018

    • Author(s)
      大堀悠美,新部邦透,江草 宏
    • Organizer
      第7回補綴若手研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel sphered culture of mesenchymeal stem cells for regenerative prosthodontics.2017

    • Author(s)
      Niibe K, Ohori-Morita Y, Zhang M, Egusa H.
    • Organizer
      International Collage of Prosthodontists
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 振盪浮遊培養を用いた間葉系幹細胞の未分化性維持方法2017

    • Inventor(s)
      新部邦透,江草 宏
    • Industrial Property Rights Holder
      新部邦透,江草 宏
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-06-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi