Role of intra and extracellular miRNAs in stem cell-based bone regeneration
| Project/Area Number |
16K20492
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | miRNA / Mesenchymal Stem Cell / Bone / Mesenchymal Stem Cells / osteoblast / MSC / bone regeneration |
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| Outline of Final Research Achievements |
Cellular regulation by microRNAs (miRNAs) in non-coding regions has recently attracted attention. miRNAs are small non-coding RNA molecules that regulates post-transcriptional gene expression. In this study, we aimed to identify miRNAs that regulate osteoblast differentiation, and analyzed miRNA expression during osteoblast differentiation of bone marrow-derived mesenchymal stem cells. In this analysis, the expression level of the miRNA was low, making it impossible to conduct reliable profiling. Since it is suggested that the purification method of miRNA and the selection of the proper normalization method are important, further analysis is ongoing now.
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| Academic Significance and Societal Importance of the Research Achievements |
miRNAs have an important role in cell metabolism, understanding the contribution of miRNAs in the osteoblastic differentiation of MSCs could provide novel and more efficient clinical protocols for patients that require bone augmentation without recurring to the current invasive standard methods.
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Report
(4 results)
Research Products
(8 results)
