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Application of DFAT cell for periodontal regeneration

Research Project

Project/Area Number 16K20519
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Prosthodontics/ Dental materials science and
Research InstitutionNihon University

Principal Investigator

AKITA Daisuke  日本大学, 歯学部, 助教 (00736879)

Research Collaborator TORIUMI Taku  
ARAI Yoshinori  
MATSUMOTO Taro  
KANO Koichiro  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords脱分化脂肪細胞 / 歯周組織再生 / 歯学 / 移植・再生医療 / 細胞・組織
Outline of Final Research Achievements

Periodontitis makes dental treatment difficult because prosthesis device becomes unstable by highly ridge absorption. Recently, mesenchymal stem cell-based approaches have been researched for regenerative medicine. However, tissue harvesting is limited. Mature adipocyte-derived dedifferentiated fat cells (DFAT) which established by ceiling culture methods are easily isolated from a small amount of adipose tissue and are homogeneous cell population with high purity. DFAT cells can be established from buccal fat, we revealed that DFAT cells facilitate periodontal regeneration in inbred rat fenestration defects. Furthermore, we investigated the periodontal regenerative potential of DFAT cells in swine as preclinical examine. In consequence, newly formed periodontal ligament, cementum and alveolar bone were observed in DAFT cells transplantation group. However, we couldn’t but change an animal to use because of easily controls rearing and the number of the suitable samples.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad2018

    • Author(s)
      Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T Kazama T, Oki Y, Saito-Tamura Y, Tonogi M, Shimizu N, Honda M
    • Journal Title

      Journal of Oral Science

      Volume: 60 Issue: 1 Pages: 14-23

    • DOI

      10.2334/josnusd.16-0786

    • NAID

      130006539176

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration2017

    • Author(s)
      Suzuki D, Akita D, Tsurumachi N, Kano K, Yamanaka K, Kaneko T, Kawano E, Iguchi S, Toriumi T, Arai Y, Matsumoto T, Sato S, Honda M
    • Journal Title

      Journal of Oral Science

      Volume: 59 Issue: 4 Pages: 611-620

    • DOI

      10.2334/josnusd.16-0878

    • NAID

      130006286520

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ransplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration.2017

    • Author(s)
      Suzuki D, Akita D, Tsurumachi N, Kano K, Katsuyuki Y, Kaneko T, Kawano K, Iguchi1 S, Toriumi T, Arai Y, Matsumoto T, Sato S, Honda M
    • Journal Title

      J Oral Sci

      Volume: in press

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of collagenase concentration to isolate small adipocytes from human buccal fat pad2017

    • Author(s)
      Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, Oki Y, Tamura Y, Tonogi N, Shimizu N, Honda M
    • Journal Title

      J Oral Sci

      Volume: in press

    • NAID

      130006539176

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Use of rat mature adipocyte-derived dedifferentiated fat cells as a cell source for periodontal tissue regeneration.2016

    • Author(s)
      Akita D, Kano K, Saito-Tamura Y, Mashimo T, Sato-Shionome M, Tsurumachi N, Yamanaka K, Kaneko T, Toriumi T, Arai Y, Tsukimura N, Matsumoto T, Ishigami T, Isokawa K, Honda M
    • Journal Title

      Frontier Physiology

      Volume: 7 Pages: 50-50

    • DOI

      10.3389/fphys.2016.00050

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] DFAT細胞と乳酸-グリコール酸共重合体(PLGA)による歯周組織再生能の検討.2016

    • Author(s)
      秋田大輔, 月村直樹, 館野 敦, 大谷賢二, 永井栄一, 石上友彦
    • Organizer
      第125回日本補綴歯科学会
    • Place of Presentation
      石川県立音楽堂(石川県金沢市)
    • Year and Date
      2016-07-08
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 脱分化脂肪細胞の取得方法2016

    • Inventor(s)
      本田雅規 鶴町仁奈 秋田大輔 外木守雄 加野浩一郎 松本太郎
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-04-01
    • Acquisition Date
      2016-08-26
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-12-27  

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