| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Oxidative stress suppressed osteoblast differentiation and matrix mineralization in which collagen fibril formation was diminished. All of the molecules well-known as regulators for collagen fibril formation were tested for alteration of gene expression after oxidative stress. The stress did not affect the expression of matrix proteins such as type V collagen and decorin, and did not that of the molecules which regulate triple helical formation of collagen molecules or hydroxylation/glycosylation of specific lysine residues of collagen. Among the enzymes which start the rection of collagen cross-linking, the stress enhanced the expression of lysyl oxidase (LOX) and LOX-like protein 1 (LOXL-1) only. Oxidative stress diminishes bone matrix through effect on molecular expression for collagen posttranslational modification.
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