Establishment of a new method for regeneration of hard tissue using stem cells

Research Project

Project/Area Number	16K20543
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Dental engineering/Regenerative dentistry
Research Institution	Showa University
Principal Investigator	Matsumoto Akifumi 昭和大学, 歯学部, 兼任講師 (50736486)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	骨 / BMP / LPS / IL-1 / TGF / 破骨細胞 / 異所性骨形成 / 再生 / 幹細胞 / 骨代謝 / 骨芽細胞 / 再生歯学 / 骨形成
Outline of Final Research Achievements	BMP (bone morphogenetic protein) induces ectopic bone formation when it was implanted into the muscle tissues. We found that TGF strongly enhances ectopic bone formation by BMP-2, while LPS inhibits it. From the analysis data, TGF increased the number of osteoblasts and chondrocytes but reduced the number of osteoclasts. On the other hand, LPS reduced the ectopic bone volume less than 50%, but it was failed in IL-1 deficient mice. In addition, IL-1 inhibited the proliferation of the cells obtained from the ectopic bone, suggesting that LPS suppresses ectopic bone formation via IL-1.