| Project/Area Number |
16K20546
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | GM-CSF / マクロファージ / TNF-α / IL-4 / チタン / 生体適合性インプラント / トレシルクロリド法 / 成長因子の固定化 / 炎症性サイトカイン / 創傷治癒促進因子 / 歯学 / タンパク質固定化技術 |
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| Outline of Final Research Achievements |
We investigated the secretion of tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) from mouse macrophages(RAW264.7) activated by GM-CSF. RAW264.7 cells were cultured on titanium (Ti) discs. Secretion of TNF-α and IL-4 was evaluated using ELISA at 24h and 48h. Cell morphologies were observed using SEM, and cell viability was accessed by an MTT assay. GM-CSF caused rough and irregular surface morphology on the macrophages and resulted in a significant difference in cell viability after 48h. TNF- α secretion significantly decreased after 48 h without GM-CSF compared with that at 24h. GM-CSF significantly increased the secretion of TNF-α after 24h and 48h. IL-4 secretion was significantly different with or without GM-CSF stimulation at 24h and 48h. There was a significant increase in IL-4 secretion 24h and 48h after GM-CSF stimulation.
These results suggest that macrophage stimulated GM-CSF may promote secretion of anti-inflammatory and pro-inflammatory cytokines on Ti.
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| Academic Significance and Societal Importance of the Research Achievements |
インプラント埋入後の創傷治癒は, 生活反応期, 創内浄化期, 組織修復期, 組織再構築期の４つのステージに分けられる. 各ステージにおいて働く細胞があり, 各細胞を活性化するサイトカインが存在する. 今回の研究では, 初期の治癒反応に関係するマクロファージとGM-CSFに狙いを絞って評価した. その結果, マクロファージをGM-CSFで刺激することにより創傷性サイトカインおよび炎症性サイトカインが促進された.
今後これらのサイトカインを組み合わせてチタン表面へ固定化し各ステージで働く細胞活性を促進することにより創傷治癒を短縮できる可能性があると考えられる.
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