Foundation of periodontal tissue regenaration using Emdogain-derived synthetic peptide from the view point of angiogenesis

• Project/Area Number: 16K20551
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: TAKAHASHI Saitatsu 大阪歯科大学, 歯学部, 講師(非常勤) (80734342)
• Research Collaborator: YAMAWAKI Isao 大阪歯科大学, 歯学部, 助教 ; TAGUCHI Yoichiro 大阪歯科大学, 歯学部, 准教授 ; UMEDA Makoto 大阪歯科大学, 歯学部, 教授
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯周病 / 再生 / 血管新生 / 歯周組織再生 / エナメルマトリックス / 結合組織 / エナメルマトリックスデリバティブ / 血管内皮細胞 / エナメル基質タンパク

Outline of Final Research Achievements

Enamel Matrix Derivative(EMD), which is commonly used as a material for periodontal tissue regeneration, a substance consistent with the partial sequence of amelogenin exon 5 was identified. We compare the experiment has compared the peptide with EMD from the view point of angiogenesis. Periodontal tissue defects were artificially prepared in the maxillary molar region of rats, and a new synthetic peptide or EMD was applied to the site of defect. Spicemens at this site were prepared 3, 5, and 7 days after surgery, and histopathologically and immunohistochemically examined using anti-type Ⅲ collagen antibody. Like EMD, novel synthetic peptide promoted the early wound healing process in the periodontal tissues compared to the control, suggesting that it has action accelerate periodontal regeneration.

Research Products

• [Journal Article] Hyperglycemia effects hard tissue formation in bone barrow mesenchymal stem cells and periodontal ligament stem cells (2017)
  • Author(s): 嘉藤 弘仁, 田口 洋一郎, 山脇 勲, 奥田 麻貴子, 小石 玲子, 野口 正皓, 山内 伸浩, 今井 一貴, 高橋 宰達, 田中 昭男, 梅田 誠
  • Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Volume: 59 Issue: 3 Pages: 118-124
  • DOI: 10.2329/perio.59.118
  • NAID: 130006105752
  • ISSN: 0385-0110, 1880-408X
  • Peer Reviewed
• [Presentation] 高グルコース環境とPorphyromonas gingivalis刺激におけるヒト骨髄間葉系細胞に対するvitamin Eの影響 (2017)
  • Author(s): 野口 正皓, 田口 洋一郎, 山脇 勲, 片山 暢仁, 高橋 宰達, 梅田 誠
  • Organizer: 日本歯科保存学会平成29年度春季学術大会