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Fundamental study on bone regeneration by allogeneic adipose-derived stem cells utilizing immunotolerance of stem cells

Research Project

Project/Area Number 16K20560
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionThe University of Tokyo

Principal Investigator

Takahashi Naoko  東京大学, 医学部附属病院, 病院診療医 (10569635)

Research Collaborator TAKATO Tshuyoshi  
HOSHI Kazuto  
KANAZAWA Sanshiro  
UTO Sakura  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords脂肪由来間葉系幹細胞 / 免疫寛容 / 次世代シーケンサー / 幹細胞特性 / 脂肪組織由来間葉系幹細胞 / 免疫抑制 / 制御性T細胞 / 歯学
Outline of Final Research Achievements

We examined the factors associated in immune tolerance by cytokine assay. However, ASC isolated by FACS was remained an issue, as these cells were heterogeneity cell population. Therefore, we verified whether there are cells involved in immune tolerance in the population using single cell RNA-Seq. As a result, ASC isolated by the conventionally method revealed that were consists of ten populations from genetic similarity. Furthermore, through global gene analysis, expression of a genes related in an immune tolerance were upregulated in a specific cell population X. When this cell population was co-cultured with CD3-positive T cells in LPS-supplemented medium, we confirmed differentiation of CD3 positive T cells to CD4/25/Foxp3 positive Treg was significantly increased. In addition, the signal existing between population X and CD3 positive T cells was analyzed by RNA-Seq. As a result, we identified candidate factors associated in the immune tolerance.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 尖端巨大症による下顎前突症に顎矯正手術を行った2例2016

    • Author(s)
      高橋直子 他
    • Journal Title

      日本顎変形症学会雑誌

      Volume: 26 Pages: 26-36

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Relation between the palatal morphology and the maxillary growth in patients with unilateral cleft lip and palate.2017

    • Author(s)
      Takahashi N et al
    • Organizer
      13th International Congress on Cleft Lip/Palate and Related Craniofacial Anomalies
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乳歯列期における顎裂部骨移植後の上顎前歯部の矯正歯科治療2017

    • Author(s)
      高橋直子 他
    • Organizer
      第41回 日本口蓋裂学会総会・学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 矯正歯科治療・顎矯正手術を行ったOpitz症候群の1例2017

    • Author(s)
      高橋直子 他
    • Organizer
      第27回日本顎変形症学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 顎関節強直症による小下顎症の1例2017

    • Author(s)
      高橋直子 他
    • Organizer
      第76回日本矯正歯科学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Evaluation of the long-term outcomes: An important role of orthodontists in the team care for craniofacial anomalies.2016

    • Author(s)
      Takahashi N et al
    • Organizer
      Asian Pacific Craniofacial Association
    • Place of Presentation
      Nara, Japan, Nara Centennial Hall
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 頭蓋顔面先天異常患者における平均顎顔面形態3Dモデル作成法の開発2016

    • Author(s)
      高橋直子 他
    • Organizer
      第75回日本矯正歯科学会大会
    • Place of Presentation
      アスティとくしま, 徳島県
    • Year and Date
      2016-11-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 口蓋裂を伴うTreacher Collins 症候群―上下顎移動術に先立ちSARMEを施行した2例2016

    • Author(s)
      高橋直子 他
    • Organizer
      第40回日本口蓋裂学会総会
    • Place of Presentation
      ナレッジキャピタル コングレコンベンションセンター, 大阪府
    • Year and Date
      2016-05-26
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-12-27  

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