Establishment and characterization of oral and maxillofacial disease-specific human iPSCs in serum-, integration- and feeder-free culture
| Project/Area Number |
16K20580
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞培養 / 再生医療 / 遺伝子疾患 / 歯学 / 再生医学 / 細胞・組織 |
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| Outline of Final Research Achievements |
In this study, we have demonstrated the generation of the disease-specific human iPSCs from oral and maxillofacial disease in completely serum-, feeder-, and integration-free culture. We have successfully generated iPSCs from PBMCs of CCD-iPSCs, Noonan syndrome, Neurofibromatosis type I, Nevoid Basal Cell Carcinoma Syndrome and Cowden syndrome patients in integration-, feeder cell-, and serum-free defined culture. In CCD- and Noonan-iPSCs, there were difference in cartilageous elements between wild type and CCD and Noonan iPSCs in chondrogenesis. This difference observed was similar to those symptoms of these patients. Using these disease-specific iPSCs would provide a powerful platform for examining the pathophysiology of relevant diseases at the cellular and molecular levels and for developing new drugs and therapeutic regimens.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Generation and maintenance of human induced pluripotent stem cells in serum-free and feeder-free culture condition-comparison of various target cells and virus vectors.2016
Author(s)
E.Akagi, S.Yamasaki, A.Hamada, H.Nakatao, F.Obayashi, T.Yasui, Y.Taguchi, M.Ohtaka, K.Nishimura, M.Nakanishi, T.Okamoto.
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Journal Title
Jpn. J. Tissue Cult. Soc. Dent. Res.
Volume: 12(1)
Pages: 21-22
Related Report
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[Journal Article] Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2015
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
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Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Issue: 2
Pages: 252-264
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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