| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we have demonstrated the generation of the disease-specific human iPSCs from oral and maxillofacial disease in completely serum-, feeder-, and integration-free culture. We have successfully generated iPSCs from PBMCs of CCD-iPSCs, Noonan syndrome, Neurofibromatosis type I, Nevoid Basal Cell Carcinoma Syndrome and Cowden syndrome patients in integration-, feeder cell-, and serum-free defined culture. In CCD- and Noonan-iPSCs, there were difference in cartilageous elements between wild type and CCD and Noonan iPSCs in chondrogenesis. This difference observed was similar to those symptoms of these patients. Using these disease-specific iPSCs would provide a powerful platform for examining the pathophysiology of relevant diseases at the cellular and molecular levels and for developing new drugs and therapeutic regimens.
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