| Project/Area Number |
16K20596
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | エナメル上皮腫 / 歯原性腫瘍 / 間質線維芽細胞 / ３次元培養 / 集団的細胞浸潤 / 三次元培養 |
|---|
| Outline of Final Research Achievements |
In order to compare the collective invasive forms of ameloblastoma cell lines AM-1 (plexiform type) and AM-3 (follicular type) of different tissue types, we performed 3D culture using Double Layered Collagen Gel Hemisphere culture (DL-CGH) method. Moreover, collective cellular invasion of ameloblastoma cells was assessed in the presence or absence of fibroblasts. Notably, without fibroblasts, AM-1 cells formed sharp, plexiform-like invasive processes, whereas AM-3 cells formed a series of blunt processes often observed during collective migration. In comparison, we could found that the presence of fibroblasts affected the invasive form of ameloblastoma cells.
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