The elucidation of the mechanism of various forms of collective cellular invasion in ameloblastoma

• Project/Area Number: 16K20596
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kagoshima University
• Principal Investigator: FUCHIGAMI Takao 鹿児島大学, 鹿児島大学病院, 助教 (40772439)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: エナメル上皮腫 / 歯原性腫瘍 / 間質線維芽細胞 / ３次元培養 / 集団的細胞浸潤 / 三次元培養

Outline of Final Research Achievements

In order to compare the collective invasive forms of ameloblastoma cell lines AM-1 (plexiform type) and AM-3 (follicular type) of different tissue types, we performed 3D culture using Double Layered Collagen Gel Hemisphere culture (DL-CGH) method. Moreover, collective cellular invasion of ameloblastoma cells was assessed in the presence or absence of fibroblasts. Notably, without fibroblasts, AM-1 cells formed sharp, plexiform-like invasive processes, whereas AM-3 cells formed a series of blunt processes often observed during collective migration. In comparison, we could found that the presence of fibroblasts affected the invasive form of ameloblastoma cells.

Research Products

• [Journal Article] Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model (2017)
  • Author(s): Fuchigami T, Koyama H, Kishida M, Nishizawa Y, Iijima M, Kibe T, Ueda M, Kiyono T, Maniwa Y, Nakamura N, Kishida S
  • Journal Title: FEBS Open Bio Volume: 7 Issue: 12 Pages: 2000-2007
  • DOI: 10.1002/2211-5463.12313
  • NAID: 120006380405
  • Peer Reviewed / Open Access
• [Presentation] THE PRESENCE OF STROMAL FIBROBLASTS AFFECT ON THE COLLECTIVE CELLULAR MIGRATION OF TUMOR CELLS IN AMELOBRASTOMA (2017)
  • Author(s): Takao Fuchigami, Shosei Kishida, Toshiro Kibe, Norifumi Nakamura
  • Organizer: 23rd International conference of oral and maxillofacial surgery
  • Place of Presentation: Hong Kong Convention & Exhibition Centre (Hong Kong, China)
  • Year and Date: 2017-03-31
  • Int'l Joint Research
• [Presentation] THE PRESENCE OF STROMAL FIBROBLASTS AFFECT ON THE COLLECTIVE CELLULAR MIGRATION OF TUMOR CELLS IN AMELOBRASTOMA (2017)
  • Author(s): T. Fuchigami, S. Kishida, T. Kibe, N. Nakamura
  • Organizer: 23rd International Conference on Oral and Maxillofacial Surgery
  • Int'l Joint Research
• [Presentation] エナメル上皮腫細胞におけるIL-1α依存性のMMP-9分泌は腫瘍細胞の浸潤に重要である (2017)
  • Author(s): 渕上貴央，岸田昭世，岐部俊郎，石畑清秀, 中村典史
  • Organizer: 第71回日本口腔科学会学術集会
• [Presentation] エナメル上皮腫の病態解明に有用な蛍光蛋白質安定発現細胞株を用いた新規三次元共培養実験系の構築 (2016)
  • Author(s): 渕上貴央、岸田昭世、岐部俊郎、中村典史
  • Organizer: 第70回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2016-04-16