Identification of novel metastatic markers using PDX model mouse on oral cancer

• Project/Area Number: 16K20611
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Dokkyo Medical University
• Principal Investigator: Kinouchi Makoto 獨協医科大学, 医学部, 助教 (00759483)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: microRNA / exosome / エクソソーム / microaRNA / PDXモデルマウス / Patient-Derived-Modelマウス / 口腔癌 / liquid biopsy / 歯学 / 腫瘍学

Outline of Final Research Achievements

I examined a pilot study before the establishment of PDX model mouse. First, I experimented using a miR-518c as the secretory microRNA by oral cancer cells, CAL-27. I isolated the exosome from their supernatant and quantified. I tried to exam the expression of miR-518c using qRT-PCR, however, the collect rare was very low. This results indicated that the following study was so hard. On the study using PDX model mouse, the collection rate of exosome from their serum was lower than from culture supernatant. Therefore, I could not acquire the result such as my hypothesis.

Academic Significance and Societal Importance of the Research Achievements

今回の研究において，口腔癌に特異的な分泌型microRNAが同定できれば，所属リンパ節転移あるいは遠隔転移の早期発見につながった可能性があるが，血清からのexosomeの収率が低く，予想したようなmiRNAの同定には至らなかった．今後の研究でさらに微量での検出を行えるような手法の開発や同定方法を確立したいと考える．

Research Products

• [Presentation] Determination of the origin of oral squamous cell carcinoma by microarray analysis (2018)
  • Author(s): Makoto Kinouchi, Sayaka Izumi, Daisuke Uchida, Nobuyuki Kuribayashi, Yuske Komiyama, Shuji Tsuchida, Atsushi Fujita, Hitoshi Kawamata.
  • Organizer: 109th AACR Annual Meeting
  • Int'l Joint Research
• [Presentation] MicroRNA-518c-5p promotes the metastasis of oral cancer (2017)
  • Author(s): Makoto Kinouchi, Daisuke Uchida, Nobuyuki Kuribayashi, Yuske Komiyama, Shuji Tsuchida, Hitoshi Kawamata
  • Organizer: 108th AACR Annual Meeting
  • Int'l Joint Research
• [Presentation] AMD070, a novel orally bioavailable CXCR4 inhibitor, inhibits the metastases of oral cancer via SDF-1/CXCR4 system (2016)
  • Author(s): Makoto Kinouchi, Daisuke Uchida, Nobuyuki Kuribayashi, Takahiro Wakui, Kyoko Kuribayashi, Maki Okubo, Masahiro Saito, Eri Masuyama, Hitoshi Kawamata
  • Organizer: 全米癌学会
  • Place of Presentation: New Orleans, Louisiana
  • Year and Date: 2016-04-16
  • Int'l Joint Research