| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We clearly demonstrated that iPSCs are capable of differentiating into a specific cell lineage in response to certain factors contained in frozen sections of tissues/organs. Interestingly, the differentiation efficiency of iPSCs on the frozen sections was clearly different among iPSC clones. Judging from these facts, this induction method of human iPSCs using frozen sections is considered to be useful as a simple and effective means for inducing the differentiation of iPSCs and also for evaluating the potential and/or quality of iPSCs. Our results also suggest that some factors, such as proteins and microRNA that can be inactivated/eliminated by fixation, may contribute to the induced differentiation of iPSCs on frozen sections. The cellular and molecular biological mechanisms underlying this phenomenon could be clearly elucidated in the near future.
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