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Time-dependent change of cellular inflammation-related response

Research Project

Project/Area Number 16K20627
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionHealth Sciences University of Hokkaido (2017)
Hokkaido University (2016)

Principal Investigator

Nishide Shinya  北海道医療大学, リハビリテーション科学部, 講師 (40451398)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords概日リズム / 蛍光イメージング / 炎症 / 生活習慣 / 時計遺伝子 / MAPK / FRET / MAPK / 小児歯科学 / 生理学
Outline of Final Research Achievements

Circadian rhythms are crucial factors in the regulation of a wide range of physiological processes including inflammation. Inflammatory responses to bacterial infection are higher during daytime in some organs in nocturnal rodents (nighttime in human), and such daily variations seem to be controlled by clock genes. However, the molecular link between clock genes and rhythms in inflammation remains to be elucidated. We analyzed intracellular activity of inflammatory markers using fluorescence microscopy with biosensors based on the principle of FRET. FRET is an energy transfer phenomenon between two different fluorescent proteins. We used biosensors which respond to inflammation and change their emitting wavelength. Reaction of biosensors to inflammatory agents displayed time dependency. Cells lacking a clock gene also responded to the drugs, but the response was not in a time independent manner. We hereby unveiled the clock gene-mediated circadian rhythms in inflammatory response.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (8 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Ebola virus requires a host scramblase for externalization of phosphatidylserine on the surface of viral particles2018

    • Author(s)
      Nanbo Asuka、Maruyama Junki、Imai Masaki、Ujie Michiko、Fujioka Yoichiro、Nishide Shinya、Takada Ayato、Ohba Yusuke、Kawaoka Yoshihiro
    • Journal Title

      PLoS Pathog

      Volume: 14 Issue: 1 Pages: e1006848-e1006848

    • DOI

      10.1371/journal.ppat.1006848

    • NAID

      120006416019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells2017

    • Author(s)
      M. Horiguchi, M. Fujioka, T. Kondo, Y. Fujioka, X. Li, K. Horiuchi, A.O. Satoh, P. Nepal, S. Nishide, A. Nanbo, T. Teshima and Y. Ohba.
    • Journal Title

      Cell Structure and Function

      Volume: 42 Issue: 1 Pages: 15-26

    • DOI

      10.1247/csf.16019

    • NAID

      130005308488

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 蛍光イメージングを利用した炎症反応の時刻依存性解析2017

    • Author(s)
      西出真也
    • Organizer
      第55回日本小児歯科学会大会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] FRETイメージングを利用した炎症反応の概日リズム解析2017

    • Author(s)
      西出真也、藤岡容一朗、堀内浩水、佐藤絢、ネパール プラバ、柏木彩花、王セイ、吉田藍子、パウデルサラド、南保明日香、大場雄介
    • Organizer
      第97回北海道医学大会生理系分科会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Profiling of time-dependency of inflammation by FRET imaging2017

    • Author(s)
      Nishide, S.
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サーカディアンリズムを考慮した口腔疾患予防2017

    • Author(s)
      西出真也
    • Organizer
      北海道医療大学歯学会特別講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Fluorescence bioimaging of intracellular dynamics between BMAL1 and CLOCK.2016

    • Author(s)
      Nishide S, Fujioka Y, Horiuchi K, Horiguchi M, Satoh AO, Nepal P, Wang J, Nanbo A, Ohba Y
    • Organizer
      第22回日本時間生物学会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
  • [Presentation] Fluorescence bioimaging of intracellular dynamics of the clock proteins.2016

    • Author(s)
      Nishide S
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
  • [Presentation] ライブセルイメージングの口腔疾患予防への応用.2016

    • Author(s)
      西出真也
    • Organizer
      第58回歯科基礎医学会学術大会サテライトシンポジウム「若手の口腔生理学研究最前線」
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 蛍光バイオセンサーを用いた時計タンパク質BMAL1-CLOCKの細胞内動態の解析.2016

    • Author(s)
      西出真也、藤岡容一朗、堀内浩水、堀口美香、佐藤絢、ネパール プラバ、王 セイ、南保明日香、大場雄介.
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都テルサ(京都市)
    • Year and Date
      2016-06-15
    • Related Report
      2016 Research-status Report
  • [Book] Biological Clocks2017

    • Author(s)
      本間 研一、本間 さと(編) 西出真也他16名(著)
    • Total Pages
      201
    • Publisher
      北海道大学出版会
    • ISBN
      9784832903722
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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