| Project/Area Number |
16K20636
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 破骨細胞 / DPP-4阻害薬 / 骨吸収 / 歯学 / 免疫 |
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| Outline of Final Research Achievements |
Tthe effect of DPP-4 inhibition on LPS-induced osteoclast formation in vivo, we subcutaneously injected LPS with or without DPP-4 inhibitor into mouse calvariae. The mean number of TRAP-positive osteoclasts in the group that underwent LPS and DPP-4 inhibitor co-administration was significantly lower than in the group that underwent LPS administration alone. TRAP and cathepsin K mRNA expression levels were significantly lower in the LPS and DPP-4 inhibitor co-administered group, compared with the LPS-administered group.In vitro, there were no direct effects of DPP-4 inhibitor or DPP-4 on RANKL- and TNF-α-induced osteoclastogenesis, or LPS-induced RANKL expression in stromal cells. Nevertheless, macrophages from LPS and DPP-4 inhibitor co-administrated mice exhibited lower TNF-α mRNA expression than macrophages from LPS-only mice. However, TNF-α mRNA expression was not reduced in LPS and DPP-4 inhibitor co-treated macrophages in vitro, compared with macrophages treated with LPS alone.
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