DPP-4 inhibitor impedes lipopolysaccharide-induced osteoclast formation and bone resorption

• Project/Area Number: 16K20636
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: ISHIDA MASAHIKO 東北大学, 歯学研究科, 助教 (80770891)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 破骨細胞 / DPP-4阻害薬 / 骨吸収 / 歯学 / 免疫

Outline of Final Research Achievements

Tthe effect of DPP-4 inhibition on LPS-induced osteoclast formation in vivo, we subcutaneously injected LPS with or without DPP-4 inhibitor into mouse calvariae. The mean number of TRAP-positive osteoclasts in the group that underwent LPS and DPP-4 inhibitor co-administration was significantly lower than in the group that underwent LPS administration alone. TRAP and cathepsin K mRNA expression levels were significantly lower in the LPS and DPP-4 inhibitor co-administered group, compared with the LPS-administered group.In vitro, there were no direct effects of DPP-4 inhibitor or DPP-4 on RANKL- and TNF-α-induced osteoclastogenesis, or LPS-induced RANKL expression in stromal cells. Nevertheless, macrophages from LPS and DPP-4 inhibitor co-administrated mice exhibited lower TNF-α mRNA expression than macrophages from LPS-only mice. However, TNF-α mRNA expression was not reduced in LPS and DPP-4 inhibitor co-treated macrophages in vitro, compared with macrophages treated with LPS alone.

Research Products

• [Journal Article] Corrosion resistance and mechanical properties of titanium nitride plating on orthodontic wires (2018)
  • Author(s): Sugisawa, H., Kitaura, H., Ueda, K., Kimura, K., Ishida, M., Ochi, Y., Kishikawa, A., Ogawa, S., Takano-Yamamoto T
  • Journal Title: Dental Materials Journal Volume: 37 Issue: 2 Pages: 286-292
  • DOI: 10.4012/dmj.2016-348
  • NAID: 130006588780
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The glucagon-like peptide-1 receptor agonist exendin-4 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption via inhibition of TNF-α expression in macrophages. (2018)
  • Author(s): Wei-Ren Shen, Keisuke Kimura, Masahiko Ishida, Haruki Sugisawa, Akiko Kishikawa, Kazuhiro Shima, Saika Ogawa, Jiawei Qi, Hideki Kitaura
  • Journal Title: Journal of Immunology Research Volume: 2018 Pages: 5783639-5783639
  • DOI: 10.1155/2018/5783639
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of muramyl dipeptide for lipopolysaccharide-mediated biological activity and osteoclast activity. (2018)
  • Author(s): Kitaura, H., Ishida, M., Kimura, K., Sugisawa, H., Kishikawa A, Shima, K., Ogawa S., Qi J, Kitaura H., Shen WR
  • Journal Title: Anal. Cell. Pathol. Volume: Volume 2018 Pages: 8047610-8047610
  • DOI: 10.1155/2018/8047610
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 歯科矯正用Ni-Tiワイヤーに対する表面コーティングによる耐食性の解析 (2017)
  • Author(s): 木村桂介，杉澤晴紀，井田裕人，石田匡彦，島和弘，岸川明子，小川紗衣香，北浦英樹
  • Journal Title: 東北矯正歯科学会雑誌 Volume: 25 Pages: 9-15
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] IL-37 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption in vivo. (2016)
  • Author(s): Saeed J, Kitaura H, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Takano-Yamamoto T
  • Journal Title: Immunol Lett. Volume: 175 Pages: 8-15
  • DOI: 10.1016/j.imlet.2016.04.004
  • Peer Reviewed / Open Access
• [Presentation] Inhibition of lipopolysaccharide-induced osteoclast formation and bone resorption in vivo by glucagon-like peptide-1 receptor agonist. (2018)
  • Author(s): Shen WR, Kimura K, Ishida M, Kishikawa A, Shima K, Ogawa S, Qi J, Ohori F, Noguchi T, Marahleh A, Kitaura H.,
  • Organizer: International Symposium for Multimodal Research and Education in IOHS-Liaison 2018
• [Presentation] 糖尿病治療薬DPP-4阻害薬による破骨細胞形成および骨吸収への影響について (2017)
  • Author(s): 石田匡彦、木村佳介、島和弘、沈威任、北浦英樹
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] Exendin-4, a GLP-1 receptor agonist, suppresses inflammation-induced osteoclast formation and bone resorption. (2017)
  • Author(s): Shen WR, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Shima K, Ogawa S., Qi J, Kitaura H.,
  • Organizer: The 12th International Workshop on Biomaterials in Interface Science.
• [Presentation] he effects of diabetic medicine exendin-4 for osteoclast formation and bone resorption. (2017)
  • Author(s): Shen WR, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Shima K, Ogawa S., Qi J, Kitaura H.,
  • Organizer: The 76th Annual Meeting of Japanese Orthodontic Society
• [Presentation] 脂肪酸受容体GPR120の破骨細胞形成および骨吸収との関連性の検討 (2017)
  • Author(s): 木村桂介、石田匡彦、島和弘、杉澤晴紀、越智由美子、岸川明子、小川紗衣香、斉嘉韋、沈威任、北浦英樹
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] 破骨細胞形成および骨吸収に対する脂肪細胞に発現するケモカインCXCL12の作用の検討 (2017)
  • Author(s): 島和弘、木村佳介、石田匡彦、杉澤晴紀、越智由美子、岸川明子、小川紗衣香、斉嘉韋、沈威任、北浦英樹
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] TiNコーティングを施した歯科矯正用ワイヤーの摩擦力の検討 (2017)
  • Author(s): 杉澤晴紀、北浦英樹、上田恭介、木村桂介、石田匡彦、越智由美子、岸川明子、小川紗衣香、山本照子
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] 破骨細胞形成および骨吸収に対するIL-37の作用の検討 (2016)
  • Author(s): 木村桂介、北浦英樹、石田匡彦、ジャファリ サイード、杉澤晴紀、越智由美子、岸川明子、セイ カイ、山本照子
  • Organizer: 第75回日本矯正学会
  • Place of Presentation: アスティー徳島（徳島県）
  • Year and Date: 2016-11-07
• [Presentation] 矯正学的歯の移動における破歯細胞形成および歯根吸収へのTNF-αの関与について (2016)
  • Author(s): 北浦英樹、木村桂介、石田匡彦、ジャファリ サイード、杉澤晴紀、越智由美子、岸川明子、セイ カイ、山本照子
  • Organizer: 第75回日本矯正学会
  • Place of Presentation: アスティー徳島（徳島県）
  • Year and Date: 2016-11-07
• [Presentation] Analysisi of friction of titanium nitride plating on orthodontic wires. (2016)
  • Author(s): Sugisawa H, Kitaura H, Ueda K, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Ogawa S, Takano-Yamamoto T.
  • Organizer: 11th Anniversary International Workshop on Biomaterials in Interface Science.Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2016
  • Place of Presentation: 蔵王ロイヤルホテル（宮城県）
  • Year and Date: 2016-08-30
  • Int'l Joint Research