Analysis for stimulation of GPR120 for osteoclast formation and orthodontic tooth movement

• Project/Area Number: 16K20637
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: Kimura Keisuke 東北大学, 大学病院, 助教 (70712909)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: GPR120 / DHA / 破骨細胞 / 破骨細胞形成 / 矯正学的歯の移動

Outline of Final Research Achievements

PBS, LPS, LPS+DHA, LPS+DHA+AH7614, and DHA were injected subcutaneously on the calvariae of wild-type mice to confirm the LPS-induced osteoclast formation and the inhibitive effect of GPR 120 signaling pathway on LPS-induced osteoclast formation. After supracalvarial injections of reagents, mice were euthanized for preparing paraffin sections of mouse calvaria. TRAP staining was then performed to evaluate osteoclast formation in the suture mesenchyme. In the LPS injection group, many TRAP positive osteoclasts were induced. On the other hand, there was a significant reduction of osteoclast number in the LPS+DHA injection group. In addition, the inhibitive effect of DHA on osteoclast formation was suppressed by selective GPR120 antagonist AH7614, which was shown in the LPS+DHA+AH7614 injection group.

Research Products

• [Journal Article] Corrosion resistance and mechanical properties of titanium nitride plating on orthodontic wires (2018)
  • Author(s): Sugisawa, H., Kitaura, H., Ueda, K., Kimura, K., Ishida, M., Ochi, Y., Kishikawa, A., Ogawa, S., Takano-Yamamoto T
  • Journal Title: Dental Materials Journal Volume: 37 Issue: 2 Pages: 286-292
  • DOI: 10.4012/dmj.2016-348
  • NAID: 130006588780
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The glucagon-like peptide-1 receptor agonist exendin-4 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption via inhibition of TNF-α expression in macrophages. (2018)
  • Author(s): Wei-Ren Shen, Keisuke Kimura, Masahiko Ishida, Haruki Sugisawa, Akiko Kishikawa, Kazuhiro Shima, Saika Ogawa, Jiawei Qi, Hideki Kitaura
  • Journal Title: Journal of Immunology Research Volume: 2018 Pages: 5783639-5783639
  • DOI: 10.1155/2018/5783639
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of muramyl dipeptide for lipopolysaccharide-mediated biological activity and osteoclast activity. (2018)
  • Author(s): Kitaura, H., Ishida, M., Kimura, K., Sugisawa, H., Kishikawa A, Shima, K., Ogawa S., Qi J, Kitaura H., Shen WR
  • Journal Title: Anal. Cell. Pathol. Volume: Volume 2018 Pages: 8047610-8047610
  • DOI: 10.1155/2018/8047610
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 歯科矯正用Ni-Tiワイヤーに対する表面コーティングによる耐食性の解析 (2018)
  • Author(s): 木村桂介，杉澤晴紀，井田裕人，石田匡彦，島和弘，岸川明子，小川紗衣香，北浦英樹
  • Journal Title: 東北矯正歯科学会雑誌 Volume: 25 Pages: 9-15
  • Peer Reviewed
• [Journal Article] IL-37 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption in vivo. (2016)
  • Author(s): Saeed J, Kitaura H, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Takano-Yamamoto T
  • Journal Title: Immunol Lett. Volume: 175 Pages: 8-15
  • DOI: 10.1016/j.imlet.2016.04.004
  • Peer Reviewed / Open Access
• [Presentation] Inhibition of lipopolysaccharide-induced osteoclast formation and bone resorption in vivo by glucagon-like peptide-1 receptor agonist. (2018)
  • Author(s): Shen WR, Kimura K, Ishida M, Kishikawa A, Shima K, Ogawa S, Qi J, Ohori F, Noguchi T, Marahleh A, Kitaura H.
  • Organizer: International Symposium for Multimodal Research and Education in IOHS-Liaison 2018
  • Int'l Joint Research
• [Presentation] Comparisons with and without retention in orthodontic relapse mouse models. (2018)
  • Author(s): Qi J, Kimura K, Ishida M, Kishikawa A, Shima K, Ogawa S, Shen WR, Ohori F, Noguchi T, Marahleh A, Kitaura H
  • Organizer: International Symposium for Multimodal Research and Education in IOHS-Liaison 2018
  • Int'l Joint Research
• [Presentation] Effect of IL-18 on mechanical loading-induced osteoclastogenesis and bone resorption. (2017)
  • Author(s): Ochi Y., Kitaura H., Kimura K ., Ishida M., Sugisawa H., Saeed J., Kishikawa A., Qi J., Takano-YAmamoto T.
  • Organizer: The 2017 Japan-NIH joint Symposium
  • Place of Presentation: 星稜オーディトリアム（宮城県）
  • Year and Date: 2017-02-15
  • Int'l Joint Research
• [Presentation] Effect of IL-18 on mechanical loading-induced osteoclastogenesis and bone resorption (2017)
  • Author(s): Ochi Y, Kitaura H, Kimura K, Ishida M, Sugisawa H, Saeed J, Kishikawa A, Qi J, Takano-Yamamoto T.
  • Organizer: The 2017 Japan-NIH joint Symposium.
  • Int'l Joint Research
• [Presentation] Exendin-4, a GLP-1 receptor agonist, suppresses inflammation-induced osteoclast formation and bone resorption (2017)
  • Author(s): Shen WR, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Shima K, Ogawa S., Qi J, Kitaura H.
  • Organizer: The 12th International Workshop on Biomaterials in Interface Science. Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2017
  • Int'l Joint Research
• [Presentation] he effects of diabetic medicine exendin-4 for osteoclast formation and bone resorption. (2017)
  • Author(s): Shen WR, Kimura K, Ishida M, Sugisawa H, Ochi Y, Kishikawa A, Shima K, Ogawa S., Qi J, Kitaura H.
  • Organizer: The 76th Annual Meeting of the Japanese Orthodontic Society.
  • Int'l Joint Research
• [Presentation] 脂肪酸受容体GPR120の破骨細胞形成および骨吸収との関連性の検討 (2017)
  • Author(s): 木村桂介、石田匡彦、島和弘、杉澤晴紀、越智由美子、岸川明子、小川紗衣香、セイ カイ、沈威任、北浦英樹
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] 破骨細胞形成および骨吸収に対する脂肪細胞に発現するケモカインCXCL12の作用の検討 (2017)
  • Author(s): 島和弘、木村桂介、石田匡彦、杉澤晴紀、越智由美子、岸川明子、小川紗衣香、セイ カイ、沈威任、北浦英樹
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] 糖尿病治療薬DPP-4阻害薬による破骨細胞形成および骨吸収への影響について (2017)
  • Author(s): 石田匡彦、木村桂介、島和弘、沈威任、北浦英樹
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] TiNコーティングを施した歯科矯正用ワイヤーの摩擦力の検討 (2017)
  • Author(s): 杉澤晴紀、北浦英樹、上田恭介、木村桂介、石田匡彦、越智由美子、岸川明子、小川紗衣香、山本照子
  • Organizer: 第76回日本矯正歯科学会大会
• [Presentation] 破骨細胞形成および骨吸収に対するIL-37の作用の検討 (2016)
  • Author(s): 木村桂介、北浦英樹、石田匡彦、ジャファリ サイード、杉澤晴紀、越智由美子、岸川明子、セイ カイ、山本照子
  • Organizer: 第75回日本矯正歯科学会大会
  • Place of Presentation: アスティ徳島（徳島県）
  • Year and Date: 2016-11-07
• [Presentation] 矯正学的歯の移動における破歯細胞形成および歯根吸収へのTNF-αの関与について (2016)
  • Author(s): 北浦英樹、木村桂介、石田匡彦、杉澤晴紀、越智由美子、岸川明子、セイ カイ、山本照子
  • Organizer: 第75回日本矯正歯科学会大会
  • Place of Presentation: アスティ徳島（徳島県）
  • Year and Date: 2016-11-07
• [Presentation] Oral health-related quality of life and perceptions of jaw dysfunction and maxillofacial asymmetry in orthodontic patients. (2016)
  • Author(s): Kitaura H., Kuroki T., Ishida M., Kimura,. Takano-Yamamoto T.
  • Organizer: The 23rd General Meeting of the Japanese Association for Dental Science.
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2016-10-21
  • Int'l Joint Research
• [Presentation] Analysis of friction oh titanium nitride plating on orthodontic wires. (2016)
  • Author(s): Sugisawa H., Kitaura H., Ueda K., Kimura K., Ishida M., Ochi Y., Kishikawa A., Ogawa S., Takano-Yamamoto T.
  • Organizer: The 11th International Workshop on Biomaterials in Interface Science Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2016.
  • Place of Presentation: バーデン家壮鳳（宮城県）
  • Year and Date: 2016-08-30
  • Int'l Joint Research