• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the progress of periodontal disease and arteriosclerosis

Research Project

Project/Area Number 16K20682
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontology
Research InstitutionAichi Gakuin University

Principal Investigator

Miyabe Megumi  愛知学院大学, 歯学部, 講師 (70734929)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords動脈硬化 / 血管リモデリング / 歯周病 / 炎症 / 歯周病態 / 診断学
Outline of Final Research Achievements

We confirmed that effects of lipopolysaharide (LPS) on regulation of human aortic smooth muscle cells (HASMCs) proliferation. Levels of DNA synthesis in HASMCs were measured by BrdU incorporation assay, and increase in the number of HASMCs were assessed by MTT-based assay. Then, we focused on toll like receptor 4 (TLR4), which was knocked down using siRNA technique. After knocking down of TRL4, LPS-stimulated proliferation of HASMCs was significantly suppressed. We also observed that LPS stimulated the migration of HASMCs, which was inhibited by the knockdown of TLR4. Furthermore, we confirmed using Western blotting that LPS stimulated the proliferation HASMCs through activation of ERK and TLR4 pathway.

Academic Significance and Societal Importance of the Research Achievements

虚血性心疾患、脳卒中など心血管疾患発症には動脈硬化が深く関与するが、動脈硬化の発生・進展には種々の危険因子が複雑に絡み合っている。危険因子として、歯周病等感染症も注目されているが、その詳細については一定の見解が得られていない。歯周病患者には、虚血性心疾患の罹患率が高いといった疫学研究が報告されているが、否定的な結果も散見される。本研究の成果より、歯周病原細菌やその産生物が直接動脈硬化を進展させる可能性が示唆された。すなわち、歯周病の予防・治療が、動脈硬化の発生・進展を抑制し、心血管疾患発症を低減させることとなり、国の施策としても揚げられている健康寿命の延伸にも寄与すると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Chemerin promotes angiogenesis in vivo.2018

    • Author(s)
      Nakamura N, Naruse K, Kobayashi Y, Miyabe M, Saiki T, Enomoto A, Takahashi M, Matsubara T.
    • Journal Title

      Physiological Reports

      Volume: 6 Issue: 24 Pages: e13962-e13962

    • DOI

      10.14814/phy2.13962

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation.2017

    • Author(s)
      3.Omi M, Hata M, Nakamura N, Miyabe M, Ozawa S, Nukada H, Tsukamoto M, Sango K, Himeno T, Kamiya H, Nakamura J, Takebe J, Matsubara T, Naruse K.
    • Journal Title

      Stem Cell Research and Therapy

      Volume: 8 Issue: 1 Pages: 279-279

    • DOI

      10.1186/s13287-017-0729-5

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Role of poly(ADP-ribose) polymerase activation in the pathogenesis of periodontitis in diabetes.2017

    • Author(s)
      Adachi K, Miyajima SI, Nakamura N, Miyabe M, Kobayashi Y, Nishikawa T, Suzuki Y, Kikuchi T, Kobayashi S, Saiki T, Mizutani M, Ohno N, Noguchi T, Mitani A, Matsubara T, Naruse K.
    • Journal Title

      J Clin Periodontol

      Volume: 44(10) Issue: 10 Pages: 971-980

    • DOI

      10.1111/jcpe.12758

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Chemerinの血管形成作用とChemerin受容体の同定2018

    • Author(s)
      中村信久、成瀬桂子、宮部愛、伊藤瑞穂、松原達昭
    • Organizer
      第50回日本動脈硬化学会総会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糖尿病性神経障害に対する歯髄幹細胞上清療法の治療効果2017

    • Author(s)
      牧野衣利子
    • Organizer
      第60回日本糖尿病学会年次学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒト歯髄幹細胞移植による糖尿病性神経障害に対する治療効果発現メカニズムの検討2017

    • Author(s)
      秦 正樹
    • Organizer
      第60回日本糖尿病学会年次学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] herapeutic effects of conditioned media from cultured dental pulp stem cells in diabetic polyneuropathy2017

    • Author(s)
      Naruse K
    • Organizer
      American Diabetes Association, 77th Scientific Sessions
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] GIPによる歯周炎抑制経路の検討2016

    • Author(s)
      鈴木佑基, 宮部愛 (他 6名、3番目)
    • Organizer
      第11回日本歯周病学会中部地区大学・日本臨床歯周病学会中部支部合同研究会
    • Place of Presentation
      朝日大学(岐阜県瑞穂市穂積)
    • Year and Date
      2016-11-03
    • Related Report
      2016 Research-status Report
  • [Presentation] GIPによる歯周炎抑制作用についての検討2016

    • Author(s)
      鈴木佑基,宮部愛 (他 16名、4番目)
    • Organizer
      第59回日本糖尿病学会年次学術集会
    • Place of Presentation
      みやこめっせ(京都府京都市左京区)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2024-12-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi