Elucidation of various signal mechanisms mediated by CSF-1 receptor of IL-34 dimer

• Project/Area Number: 16K20685
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Hyogo Medical University
• Principal Investigator: KAWABE MUTSUKI 兵庫医科大学, 医学部, 助教 (10760720)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: IL-34 / CSF-1 / CSF-1R / 破骨細胞 / 歯根膜細胞 / 再生医学

Outline of Final Research Achievements

We stimulated periodontal ligament (PDL) cells and gingival fibroblast (GF) cells by tumor necrosis factor-α (TNF-α), and evaluated the mRNAs expression of IL-34 and CSF-1 by Realtime PCR. As the result, the expression of IL-34 and CSF-1 were increased by TNF- α in PDL cells and GF cells. We got mRNA from infected granulation tissues of patients with periodontal disease, and evaluated the expression of mRNAs by Realtime PCR. The correlation between inflammatory cytokines such as TNF-α, IFN-γ and IL-1β and osteoclastogenesis factors such as IL-34 and CSF-1 was not confirmed.