Compositional analysis in oral and gut microbiota

• Project/Area Number: 16K20692
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: Ogawa Taiji 大阪大学, 歯学研究科, 助教(常勤) (10543481)
• Research Collaborator: KAWABATA SHIGETADA 大阪大学, 大学院歯学研究科, 教授 (50273694) ; MAEDA YOSHINOBU 大阪大学, 大学院歯学研究科, 特任教授 (10144510) ; IKEBE KAZUNORI 大阪大学, 大学院歯学研究科, 准教授 (70273696) ; OGAWA MARIKO 大阪大学, 大学院歯学研究科, 特任研究員 (20754732) ; HIROSE YUJIRO 大阪大学, 大学院歯学研究科, 特任助教 (90788407)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔細菌叢 / 腸内細菌叢 / 16S rRNAシークエンス解析 / 食品栄養摂取 / マイクロバイオーム / 腸内細菌 / 口腔内細菌

Outline of Final Research Achievements

We investigated the salivary microbiota composition in samples from healthy and frail elderly individuals using 16S rRNA sequencing analysis for characterization. We found a significant difference in diversity between elderly individuals living in a nursing home (EN) and healthy control (HC) subjects, as well as in the microbiota composition at the phyla level. A supervised orthogonal partial least squared discriminant analysis (OPLS-DA) revealed a significant difference in clear classification trend between the EN and HC groups, with all observations falling within the Hotellings T2 (0.95) ellipse, with model fitness parameters of R2(cum)=0.937 and Q2(cum)=0.888, respectively. In addition, the score plots by unsupervised principal component analysis (PCA) showed a clear classification trend in both groups. Our findings suggest that general frailty is associated with oral microbiota composition and formation.

Research Products

• [Journal Article] Composition of salivary microbiota in elderly subjects (2018)
  • Author(s): Ogawa Taiji、Hirose Yujiro、Honda-Ogawa Mariko、Sugimoto Minami、Sasaki Satoshi、Kibi Masahito、Kawabata Shigetada、Ikebe Kazunori、Maeda Yoshinobu
  • Journal Title: Sci Rep Volume: 8 Issue: 1 Pages: 414-414
  • DOI: 10.1038/s41598-017-18677-0
  • Peer Reviewed / Open Access
• [Journal Article] Characterizations of oral microbiota in elderly nursing home residents with diabetes: a pilot study. (2017)
  • Author(s): Ogawa T, Ogawa HM, Ikebe K, Notomi Y, Iwamoto Y, Shirobayashi I, Hata S, Kibi M, Masayasu S, Sasaki S, Kawabata S, Maeda Y
  • Journal Title: Journal of Oral Science Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 口腔細菌叢から健康を考える －口腔は腸管に次ぐ細菌叢の宝庫－ (2017)
  • Author(s): 小川 泰治，広瀬 雄二郎，本多 真理子
  • Journal Title: Medical Science Digest Volume: 43 Pages: 5-7
• [Presentation] 自立高齢者と要介護高齢者における口腔細菌叢の比較 (2017)
  • Author(s): 小川泰治，吉備政仁，池邉一典，前田芳信
  • Organizer: 日本老年歯科医学会学術大会
• [Presentation] The Mouth Matters: Characterization of Oral Microbiota in Nursing Home Residents (2017)
  • Author(s): Taiji Ogawa, Mariko Honda, Kazunori Ikebe, Masahito Kibi, Shigetada Kawabata, Yoshinobu Maeda
  • Organizer: International Association of Gerontology and Geriatrics
  • Int'l Joint Research