| Project/Area Number |
16K20692
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
Ogawa Taiji 大阪大学, 歯学研究科, 助教(常勤) (10543481)
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| Research Collaborator |
KAWABATA SHIGETADA 大阪大学, 大学院歯学研究科, 教授 (50273694)
MAEDA YOSHINOBU 大阪大学, 大学院歯学研究科, 特任教授 (10144510)
IKEBE KAZUNORI 大阪大学, 大学院歯学研究科, 准教授 (70273696)
OGAWA MARIKO 大阪大学, 大学院歯学研究科, 特任研究員 (20754732)
HIROSE YUJIRO 大阪大学, 大学院歯学研究科, 特任助教 (90788407)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 口腔細菌叢 / 腸内細菌叢 / 16S rRNAシークエンス解析 / 食品栄養摂取 / マイクロバイオーム / 腸内細菌 / 口腔内細菌 |
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| Outline of Final Research Achievements |
We investigated the salivary microbiota composition in samples from healthy and frail elderly individuals using 16S rRNA sequencing analysis for characterization. We found a significant difference in diversity between elderly individuals living in a nursing home (EN) and healthy control (HC) subjects, as well as in the microbiota composition at the phyla level. A supervised orthogonal partial least squared discriminant analysis (OPLS-DA) revealed a significant difference in clear classification trend between the EN and HC groups, with all observations falling within the Hotellings T2 (0.95) ellipse, with model fitness parameters of R2(cum)=0.937 and Q2(cum)=0.888, respectively. In addition, the score plots by unsupervised principal component analysis (PCA) showed a clear classification trend in both groups. Our findings suggest that general frailty is associated with oral microbiota composition and formation.
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