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Evolution of the neural sex-determination system in insects

Research Project

Project/Area Number 16K20875
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Animal physiology/Animal behavior
Developmental biology
Research InstitutionHokkaido University

Principal Investigator

Watanabe Takayuki  北海道大学, 理学研究院, 学術研究員 (70547851)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords性決定 / 性的二型神経回路 / doublesex / fruitless / 昆虫 / 進化 / 神経科学 / 発生・分化
Outline of Final Research Achievements

Sexually dimorphism in the brain of the fruit fly is regulated by two transcription factor genes, fruitless and doublesex. In the fly brain, these genes are expressed as sex-specific isoforms to shape sexually dimorphic circuits. To reveal the evolutionary origin of neural sex determination system in insects, identification and characterization of these two genes was conducted in basal direct-developing insects including the two-spotted cricket. The result indicated that the fruitless gene of basal direct-developing insects does not possess molecular mechanisms for generating sex-specific gene product. On the other hand, the cricket brain expresses sex-dependent gene products of doublesex. These results support the idea that the doublesex gene is a prototypic neural sex-determination gene in basal direct-developing insects, and advanced holometabolous insects acquired an additional sex-determination mechanism regulated by the fruitless gene.

Academic Significance and Societal Importance of the Research Achievements

我々ヒトを含む多くの動物は、脳の形態や脳を構成する神経回路に雌雄差(性的二型)が存在する。本研究では昆虫を材料に、性的二型が作られる遺伝的メカニズムと脳の発達メカニズム、そしてその進化を理解するための研究を展開した。本研究の結果、モデル生物であるショウジョウバエ脳で主要な神経回路の雌雄差を決定する遺伝子であるfruitless遺伝子が、コオロギやゴキブリなどの原始的な不完全変態昆虫脳においては性決定に関与しないことなど、昆虫脳における性決定メカニズムの多様性を示す結果を得た。脳の性的二型は性行動の発現に重要だと考えられることから、本研究から昆虫における性行動の神経基盤の多様性が示唆された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Immediate-Early Promoter-Driven Transgenic Reporter System for Neuroethological Research in a Hemimetabolous Insect2018

    • Author(s)
      Takayuki Watanabe, Atsushi Ugajin and Hitoshi Aonuma
    • Journal Title

      eNeuro

      Volume: 5 Issue: 4 Pages: 0061-18

    • DOI

      10.1523/eneuro.0061-18.2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 昆虫性分化機構の進化を探る:fruitless 遺伝子は原始的な昆虫で脳の性分化に関わるか?2019

    • Author(s)
      渡邊崇之
    • Organizer
      日本動物学会北海道支部第63回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 昆虫脳性決定機構の進化を探る~doublesex 遺伝子はコオロギ脳の性を決めるのか?2018

    • Author(s)
      渡邊崇之、水波誠
    • Organizer
      日本動物学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Whole-brain activity mapping for neuroethological study in the cricket2017

    • Author(s)
      Takayuki Watanabe, Atsushi Ugajin, Hitoshi Aonuma
    • Organizer
      The 8th International Symposium on Adaptive Motion of Animals and Machines (AMAM2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 初期応答遺伝子プロモーターを利用したコオロギ脳機能マッピング2017

    • Author(s)
      渡邊 崇之、宇賀神 篤、青沼 仁志
    • Organizer
      日本動物学会第88回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Whole-brain activity mapping using the immediate-early promoter-driven reporter system in the cricket2017

    • Author(s)
      Takayuki Watanabe, Atsushi Ugajin, Hitoshi Aonuma
    • Organizer
      Society for Neuroscience 2017 Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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