The relationship between porcine seminal plasma proteins and immune response on uterine affecting in pig conception

Research Project

Project/Area Number	16K20884
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Animal production science Veterinary medical science
Research Institution	Oita University
Principal Investigator	OKUYAMA Minami 大分大学, 医学部, 助教 (50756781)
Project Period (FY)	2016-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	豚 / 受胎 / 精漿 / 免疫 / 子宮内膜 / PBMCs / 獣医学 / 畜産学 / 応用動物
Outline of Final Research Achievements	This study focused on roles of seminal plasma proteins and immune response of uterine epithelial cells in order to reveal how porcine seminal plasma affect conception. This study goal is to improve pig reproduction performance with using seminal plasma. First, the methods of filtration of seminal plasma was established. Second, uterine epithelial cell isolation and culture were established. Third, cytokine mRNA expressions were measured in culture cells incubated with 10% seminal plasma medium. As the results, it is clarified that low-molecular substances in seminal plasma affected IL-8 and COX-2 expression in epithelial cells, on the other hand, did not influence IL-6 expression. Additionally, IL-1βexpression might be affected by proteins without presence of complements.
Academic Significance and Societal Importance of the Research Achievements	国内でのブタ研究は飼養管理や衛生管理に主眼が置かれており、基礎繁殖学的研究は立ち遅れている。本研究の結果は、ブタの精漿が子宮の免疫動態に及ぼす効果とその機序を知る足がかりとなり、基礎繁殖学分野の発展に寄与する。また、母体の子宮において受胎の成立と失敗をわける原因因子を特定することで、不妊の治療法の開発につなげることができるなど獣医学的意義も深い。さらに本研究を発展させ、精漿が本来もつ子宮の機能調節作用を利用した新たな繁殖促進技術の開発につなげることが期待できる。