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Mechanism of hypothalamic Corticotropin Releasing factor regulation by Leptin

Research Project

Project/Area Number 16K20891
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Endocrinology
Environmental physiology(including physical medicine and nutritional physiology)
Research InstitutionHirosaki University (2017-2019)
Tohoku University (2016)

Principal Investigator

Yamagata Satoshi  弘前大学, 医学部附属病院, 助教 (50769940)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
KeywordsLeptin / CRF / レプチン / CRFニューロン / 基礎医学 / ストレス
Outline of Final Research Achievements

In the hole cell patch-clamp recording, Venus-positive neurons in the PVH of CRF-Venus mice have glutamatergic and GABAergic inputs. In a study using CRF neuron-selective ChR2-expressing mice (ChR2-mCherry / CRF-iCreΔNeo), almost all CRF neurons expressed ChR2(mCherry). CRF neurons were depolarized and fired by blue light. Blue light irradiation of CRF neurons increased c-Fos expression in the PVH and other brain regions.

Academic Significance and Societal Importance of the Research Achievements

コルチコトロピン放出因子(CRF)はストレス応答における視床下部-下垂体-副腎軸の要であると同時に,摂食に関与すると言われている.しかし詳細なメカニズムは解明されていない.またレプチンによるCRFニューロンの調節機構は明らかになっていない.CRF-VenusΔNeoマウスは, CRFニューロンが蛍光可視化されるため電気生理学的手法や形態学的検証を可能にし,CRFニューロンのはたらきを解明するための重要なツールとなる.

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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