Elucidation of genome stabilization mechanism involving RNA editing enzyme ADAR1
Project/Area Number |
16K20895
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Risk sciences of radiation and chemicals
Tumor biology
|
Research Institution | Hirosaki University |
Principal Investigator |
|
Research Collaborator |
Nishikura Kazuko
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | RNA編集 / ゲノム不安定化 / DNA損傷 / ゲノム安定化 / ADAR1 / DNA二重鎖切断 / ゲノム不安定性 / DNA損傷修復 / 遺伝子 / ゲノム |
Outline of Final Research Achievements |
The purpose of this study is to investigate the cross-talk function of RNA editing and DNA repair mechanism by examining how ADAR1 protein with RNA editing function is involved in genome stabilization. As a result, we found that ADAR1 protein accumulates at the DNA double-strand break site, and furthermore, we observed higher radiosensitivity of cells after knock down of ADAR1 expression. ADAR1 suppression also showed a delay of DNA damage repair. These results suggest that ADAR1 protein plays an important role in DNA damage repair.
|
Academic Significance and Societal Importance of the Research Achievements |
DNA修復機構の破綻は細胞のがん化と密接な関連を持つことから、細胞がん化にADAR1の機能不全が影響している事が考えられ、得られた結果から、細胞のがん化とRNA 編集機構の接点、およびRNA編集機構によるがん抑制機構の発見への可能性が考えられる。
|
Report
(4 results)
Research Products
(3 results)