Development of diagnostic method for Niemann-Pick disease type C based on efficient analysis of lipid profile in body fluid
| Project/Area Number |
16K20900
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
Pediatrics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Research Collaborator |
NARITA Aya
Mashima Ryuichi
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ニーマンピック病C型 / コレステロール代謝物 / LC/MS/MS / 化学診断 / バイオマーカー / フォーカスドメタボロミクス / 誘導体化 / 診断マーカー / LC/ESI-MS/MS / メタボロミクス / 質量分析 / クロマトグラフィー / 臨床 |
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| Outline of Final Research Achievements |
For the purpose of developing a novel diagnostic method for Niemann-Pick disease type C, which is a lipid abnormal disease that was desired to develop a novel diagnostic method, various investigations were conducted. MS/MS conditions of various cholesterol metabolites and sphingolipids were investigated, and characteristic fragment patterns were found. In addition, LC conditions, which can identify them, were optimized. By focused metabolomics using these conditions, we found characteristic molecules in body fluids of Niemann-Pick disease type C patients. In next, we investigated the accurate analysis method for these metabolites. As a result, we developed an analytical method useful for diagnosis and found that the metabolites have very excellent diagnostic marker performances.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Identification of novel bile acids as biomarkers for the early diagnosis of Niemann‐Pick C disease2016
Author(s)
F.Mazzacuva, P.Mills, K.Mills, S.Camuzeaux, P.Gissen, E-R Nicoli, C.Wassif, D.Vruchte, F.D.Porter, M.Maekawa, N.Mano, T.Iida, F.Platt, P.T.Clayton.
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Journal Title
FEBS Letters
Volume: 590
Issue: 11
Pages: 1651-1662
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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