Analyzing of microbiome of a poor understanding group 'Ktedonobacteria' with Actinomycetes-Like Morphology and exploration of new bioactive compounds from the group.

• Project/Area Number: 16K20903
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology; Natural medicines
• Research Institution: Tohoku University
• Principal Investigator: Yabe Shuhei 東北大学, 農学研究科, 准教授 (60564838)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: クテドノバクテリア / 二次代謝産物 / 二次代謝物

Outline of Final Research Achievements

We have research on Ktedonobacteria based on a point of view that they have the potential to become a new sustainable source from which new and improved drugs can be produced, as has historically been the case with actinomycetes. As results of this study, it was revealed that Ktedonobacteria lives diversely at low abundances in common environments. And we were succeeded in isolating several diverse ktedonobacterial strains from various environments using selective medium that we developed, and we are proposing them as two new genera and six novel species in the family Ktedonobacteria. Furthermore, unique anthraquinone compounds having anti-MRSA activity was found from culture liquid extracts of the ktedonobacterial strains.
The exploitation of this group can create new developments in the field of natural products exploration by the traditional methods and may contribute to the prevention of spread of infectious diseases.

Research Products

• [Journal Article] Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus Thermosporothrix. (2017)
  • Author(s): Yabe S., Y. Sakai, K. Abe, A. Yokota, A. Také, A. Matsumoto, A. Sugiharto4, D. Susilowati, M. Hamada, K. Nara, I.M. Sudiana, S. Otsuka
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Volume: 67 Issue: 8 Pages: 2615-2621
  • DOI: 10.1099/ijsem.0.001985
  • Peer Reviewed
• [Journal Article] Diversity of <i>Ktedonobacteria</i> with Actinomycetes-Like Morphology in Terrestrial Environments (2017)
  • Author(s): Shuhei Yabe, Yasuteru Sakai, Keietsu Abe, Akira Yokota
  • Journal Title: Microbes and Environments Volume: 32 Issue: 1 Pages: 61-70
  • DOI: 10.1264/jsme2.ME16144
  • NAID: 130005519589
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Thermosporothrix narukonensis sp. nov., belonging to the class Ktedonobacteria, isolated from fallen leaves on geothermal soil, and emended description of the genus Thermosporothrix (2016)
  • Author(s): Shuhei Yabe, Yasuteru Sakai, Akira Yokota
  • Journal Title: nternational Journal of Systematic and Evolutionary Microbiology Volume: in press Issue: 6 Pages: 2152-2157
  • DOI: 10.1099/ijsem.0.001004
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 「クテドノバクテリア（綱）」のゲノム解析が明かにした新規二次代謝産物生合成の潜在能力 (2018)
  • Author(s): 鄭 宇、豊田 敦、水口 洋平、王 瓊渼、酒井 康輝、阿部 敬悦、横田 明、矢部 修平
  • Organizer: 日本農芸化学会2018年度 年次大会
• [Presentation] クテドノバクテリア綱の新しい系統に属する中温性細菌の分離と系統分類 (2017)
  • Author(s): ○矢部修平、酒井康輝、王 瓊渼、鄭宇、浜田盛之、大塚重人、阿部敬悦、横田明
  • Organizer: ２０１６年度日本農芸化学会
  • Place of Presentation: 京都女子大学（京都府京都市）
  • Year and Date: 2017-03-19
• [Presentation] コンポストから分離された次世代放線菌「クテドノバクテリア」の魅力 (2017)
  • Author(s): 矢部修平
  • Organizer: 2017年度日本生物工学会北日本支部 札幌シンポジウム「生物プロセスによるモノづくり・資源循環を目指して」
  • Invited
• [Presentation] 好熱性クテドノバクテリア（Thermogemmatisporaceae科）の分離と系統分類 (2017)
  • Author(s): 鄭 宇、王 瓊渼、酒井 康輝、阿部 敬悦、横田 明、矢部 修平
  • Organizer: 2017年度 環境微生物系合同大会
• [Presentation] Ktedonobacterales目の新しい系統に属する中温性細菌の分類 (2017)
  • Author(s): 王 瓊渼、鄭 宇、酒井 康輝、阿部 敬悦、横田 明、矢部 修平
  • Organizer: 2017年度 環境微生物系合同大会