Transition of the circuit usage for memory processing

• Project/Area Number: 16K20919
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience; Animal physiology/Animal behavior
• Research Institution: Nagoya City University (2017-2018); Tohoku University (2016)
• Principal Investigator: Ichinose Toshiharu 名古屋市立大学, 大学院薬学研究科, 研究員 (20774748)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 記憶学習 / ショウジョウバエ / キノコ体出力神経 / ドーパミン / キノコ体 / 連合学習 / 神経回路 / 短期記憶 / 長期記憶 / 記憶の読み出し回路

Outline of Final Research Achievements

The mushroom body (MB) of Drosophila melanogaster is functionally pleiotropic. However, circuit mechanisms that generate this pleiotropy are largely unclear. Here, we systematically probed the behavioral requirement of each type of MB output neurons (MBON) for appetitive or aversive memories. We evaluated the requirement using two conditioned responses: memory-guided odor choice and odor source attraction. Quantitative analysis revealed that these conditioned odor responses are controlled by distinct sets of MBONs. We found that the valence of memory, rather than the transition of memory steps, has a larger impact on the MB output patterns. Our results provide principles how the MB processes associative memories of different valence and distinct memory-guided behaviors.

Academic Significance and Societal Importance of the Research Achievements

海馬や扁桃体といった脳の高次構造は一般的に、記憶、場所情報や感情といった様々な情報を処理する。しかし、そのような多機能性がどのように実現されているか、その詳細は不明である。ショウジョウバエのキノコ体も同様に、様々な記憶情報を処理する。キノコ体を構成する神経細胞は、そのほぼ全てが明らかになっており、一細胞レベルで機能操作することができるため、キノコ体をモデルに多機能性の仕組みを解き明かすことができる。本研究は、この全ての細胞を対象に、報酬記憶と罰記憶に基づいた様々な記憶発現を行う神経回路を網羅的に同定した。この成果により、多機能性を実現する神経回路構造の詳細を明らかにすることができた。

Research Products

• [Journal Article] Behavioral modulation by spontaneous activity of dopamine neurons. (2017)
  • Author(s): Toshiharu Ichinose, Hiromu Tanimoto, Nobuhiro Yamagata
  • Journal Title: Frontiers Systems Neuroscience Volume: 11 Pages: 88-88
  • DOI: 10.3389/fnsys.2017.00088
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spontaneous activity of dopamine neurons and its role in behavioral regulation (2017)
  • Author(s): 市之瀬 敏晴, 山方 恒宏
  • Journal Title: Hikaku seiri seikagaku(Comparative Physiology and Biochemistry) Volume: 34 Issue: 4 Pages: 108-115
  • DOI: 10.3330/hikakuseiriseika.34.108
  • NAID: 130006847566
  • ISSN: 0916-3786, 1881-9346
  • Peer Reviewed
• [Journal Article] Dynamics of memory-guided choice behavior in <i>Drosophila</i> (2016)
  • Author(s): Rohwedder A, Wenz NL, Stehle B, Huser A, Yamagata N, Zlatic M, Truman JW, Tanimoto H, Saumweber T, Gerber B.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 92 Issue: 8 Pages: 346-357
  • DOI: 10.2183/pjab.92.346
  • NAID: 130005420972
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct neural pathways convey distinct visual information to Drosophila mushroom bodies. (2016)
  • Author(s): Vogt K, Aso Y, Hige T, Knapek S, Ichinose T, Friedrich AB, Turner GC, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: in press
  • DOI: 10.7554/elife.14009
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Chronic poor condition enhances preference for rewarding substances (2018)
  • Author(s): Ichinose T, Kanno M, Abe A, Tanimoto H.
  • Organizer: 3th Japanese Drosophila Research Conference
  • Int'l Joint Research
• [Presentation] Chronic poor condition enhances preference for rewarding substances through dopamine system (2018)
  • Author(s): Ichinose T, Kanno M, Kondo S, Hiramatsu S, Abe A, Tanimoto H.
  • Organizer: Asia-pacific Drosophila neurobiology conference
  • Int'l Joint Research
• [Presentation] Visualization and neuronal control of memory-guided choice behaviour. (2017)
  • Author(s): Kanno M, Ichinose T, Onodera Y, Sirigrivatanawong P, Hashimoto K, Yamagata N, Tanimoto H.
  • Organizer: Janelia Farm Conference “Structure and Function of the Insect Mushroom Body”
  • Place of Presentation: Janelia Research Campus, Verginia, USA
  • Year and Date: 2017-03-05
  • Int'l Joint Research
• [Presentation] Neural circuits that distinguish memory processes in the fly brain (2017)
  • Author(s): Toshiharu Ichinose, Mai Kanno, Hiromu Tanimoto
  • Organizer: 15th European Symposium for insect Taste and Olfaction (15th ESITO)
  • Int'l Joint Research
• [Presentation] Neural circuits that distinguish memory processes in the fly brain (2017)
  • Author(s): Toshiharu Ichinose, Mai Kanno, Hiromu Tanimoto
  • Organizer: 4th Asia-Pacific Drosophila Research Conference (APDRC4)
  • Int'l Joint Research