| Project/Area Number |
16K20928
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Collagenous pathology/Allergology
Pathobiological dentistry/Dental radiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 抗原特異的治療法 / シェーグレン症候群 / M3ムスカリン作働性アセチルコリン受容体 / 抗原特異的T細胞 / M3ムスカリン作働性受容体 |
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| Outline of Final Research Achievements |
Sjogren's syndrome (SS) is an autoimmune disease characterized by infiltration of lymphocytes including CD4+ T cells into salivary glands. The restricted usage of T cell receptor (TCR) has been reported, suggesting the antigen-specific immune response is the main pathologic mechanism. M3 muscarinic acetylcholine receptor (M3R) is evaluated to be one of the auto-antigens of SS, so we tried to evaluate M3R recative CD4 T cells from patients.
CD4+ T cells from salivary glands of a patient with primary SS were single-cell sorted and clones were established. The TCR Vβ sequences among established clones were checked. Next, functional dendritic cells were induced from CD14+ cells of the same patient. Now the evaluation of M3R reactivity of established T cell clones are going on, using dendritic cells and M3R peptides.
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