Functional analysis of DNAM-1 in Treg and investigation for therapeutic applications
| Project/Area Number |
16K20935
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
Gastroenterology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Kanemaru Yumi 筑波大学, 医学医療系, 助教 (00708688)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 制御性T細胞 / DNAM-1 / TIGIT / 炎症性腸疾患 / CD155 / 免疫学 |
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| Outline of Final Research Achievements |
In this study, the role of DNAM-1 in regulatory T cell (Treg) was investigated. In vitro experiments showed that DNAM-1-deficient Treg function was normal. On the other hand, in vivo experiments showed that DNAM-1-deficient Treg function was enhanced. By reporter cell system, it was demonstrated that DNAM-1 interfered with the binding between TIGIT and its ligand and limited Treg function. Thus, it is strongly suggested that in vivo environment is involved in the molecular mechanisms of DNAM-1-TIGIT axis in Treg. This should be a pivotal mechanism how Treg cells efficiently work in vivo (inflammatory) situation.
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| Academic Significance and Societal Importance of the Research Achievements |
過剰な免疫応答や炎症反応を抑制する細胞の１つである制御性T細胞は、自己免疫疾患や炎症性疾患の治療に有用であることが多くの研究から明らかになっている。制御性T細胞の機能を左右する因子の１つとして細胞表面に存在する受容体タンパク質が挙げられ、本研究で着目したDNAM-1もその１つである。本研究ではDNAM-1が制御性T細胞の機能を弱めてしまっていることを示した。このことは大きな学術的発見であると同時に、制御性T細胞を用いた治療においてDNAM-1を標的とする新たな治療法の可能性を示した点で社会的意義のある研究成果といえる。
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Report
(3 results)
Research Products
(5 results)
