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Elucidation of the mechanism of plant oxalate accumulation on the basis of wide targeted metabolomics

Research Project

Project/Area Number 16K20953
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Applied molecular and cellular biology
Research InstitutionSaitama University

Principal Investigator

Atsuko Miyagi  埼玉大学, 理工学研究科, 助教 (00645971)

Research Collaborator Kawai Maki (Yamada Maki)  
Yamaguchi Masatoshi  
Ishikawa Toshiki  
Nagano Minoru  
Oono Yutaka  
Hase Yoshihiro  
Ookawa Taiichiro  
Adachi Shunsuke  
Noguchi Ko  
Tokida Takeshi  
Usui Yasuhiro  
Nakamura Hirofumi  
Sakai Hidemitsu  
Hasegawa Toshihiro  
Yamamoto Toshio  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsシュウ酸 / イネ / メタボローム解析 / CE-QQQ-MS / ICL / 染色体部分置換系統 / イネ品種間比較 / 重イオンビーム照射 / メタボロミクス / 重イオンビーム / 品種間差 / イオンビーム照射
Outline of Final Research Achievements

Oxalate is accumulated in leaves of various plant species. Excess uptake of oxalate is harmful for human and livestock. However, the mechanism of oxalate accumulation remains unknown. To elucidate the mechanism of oxalate accumulation in rice, metabolome and molecular genetic analysis were performed. The results showed that isocitrate lyase (ICL) might involve in oxalate synthesis in the submerged leaves. Metabolome analysis of low oxalate-plants obtained from ion beam-irradiated rice revealed that the decrease of other organic acids would lead low oxalate content. Moreover, it was found that oxalate content in Takanari leaves is markedly lower than those in Koshihikari ones, suggesting that the difference of oxalate content between two cultivars is caused by particular chromosomal region.

Academic Significance and Societal Importance of the Research Achievements

植物のシュウ酸合成に関わる遺伝子の1つであるICLの機能解明が進んだ。また、品種間でシュウ酸含有量が著しく異なる品種が見出され、環境要因に左右されないことも明らかになった。これらの染色体部分置換系統を用いた解析を行えば、シュウ酸蓄積に寄与する、ICL以外の遺伝子についての特定が期待できる。さらに、イオンビーム照射イネ系統から見出された低シュウ酸イネ個体において、その次世代の中からも低シュウ酸形質を示すものが得られたことから、放射線育種による低シュウ酸イネの作出に近づいた。これらの知見は、稲わらのほか、ホウレンソウなどの高シュウ酸作物の品質向上につながることが期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (14 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Oxalate contents in leaves of two rice cultivars grown at a free-air CO<sub>2</sub> enrichment (FACE) site2019

    • Author(s)
      Atsuko Miyagi, Ko Noguchi, Takeshi Tokida, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Toshihiro Hasegawa, Maki Kawai-Yamada
    • Journal Title

      Plant Production Science

      Volume: - Issue: 3 Pages: 407-411

    • DOI

      10.1080/1343943x.2019.1598272

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 68032019

    • Author(s)
      Ishikawa Y., Miyagi A., Ishikawa T., Nagano M., Yamaguchi M., Hihara Y., Kaneko Y., Kawai-Yamada M.
    • Journal Title

      Plant J.

      Volume: - Issue: 4 Pages: 654-666

    • DOI

      10.1111/tpj.14262

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolic and biochemical responses of Potamogeton anguillanus Koidz. (Potamogetonaceae) to low oxygen conditions2019

    • Author(s)
      Mahfuza Parveen, Atsuko Miyagi, Maki Kawai-Yamada, Md H. Rashid and Takashi Asaeda
    • Journal Title

      Journal of Plant Physiology

      Volume: 232 Pages: 171-179

    • DOI

      10.1016/j.jplph.2018.11.023

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 68032018

    • Author(s)
      Yuta Kodama, Akihito Kawahara, Atsuko Miyagi, Toshiki Ishikawa, Maki Kawai-Yamada, Yasuko Kaneko, Yasushi Takimura and Yukako Hihara
    • Journal Title

      Biotechnology and Bioengineering

      Volume: 115 Issue: 12 Pages: 2974-2985

    • DOI

      10.1002/bit.26842

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ferredoxin/thioredoxin system plays an important role in the chloroplastic NADP status of Arabidopsis2018

    • Author(s)
      Shin‐nosuke Hashida, Atsuko Miyagi, Maho Nishiyama, Keisuke Yoshida, Toru Hisabori, Maki Kawai‐Yamada
    • Journal Title

      The Plant Journal

      Volume: 95 Issue: 6 Pages: 947-960

    • DOI

      10.1111/tpj.14000

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of metabolic changes in oxalate-rich plant Rumex obtusifolius L. caused by ion beam irradiation2018

    • Author(s)
      Miyagi Atsuko、Kitano Sayaka、Oono Yutaka、Hase Yoshihiro、Narumi Issay、Yamaguchi Masatoshi、Uchimiya Hirofumi、Kawai-Yamada Maki
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 122 Pages: 40-45

    • DOI

      10.1016/j.plaphy.2017.11.001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of elevated atmospheric CO2 on respiratory rates in mature leaves of two rice cultivars grown at a free-air CO2 enrichment site and analyses of the underlying mechanisms.2018

    • Author(s)
      Ko Noguchi, Tomonori Tsunoda, Atsuko Miyagi, Maki Kawai-Yamada, Daisuke Sugiura, Shin-Ichi Miyazawa, Takeshi Tokida, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Toshihiro Hasegawa
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Issue: 3 Pages: 637-649

    • DOI

      10.1093/pcp/pcy017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] CE-MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics2017

    • Author(s)
      Mayumi Sato, Atsuko Miyagi, Shozo Yoneyama, Seiki Gisusi, Yoshihiko Tokuji, Maki Kawai-Yamada
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 81 Issue: 12 Pages: 2314-2322

    • DOI

      10.1080/09168451.2017.1387049

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synergistic effects of light quality, carbon dioxide and nutrients on metabolite compositions of head lettuce under artificial growth conditions mimicking a plant factory2017

    • Author(s)
      Miyagi A, Uchimiya H, Kawai-Yamada M
    • Journal Title

      Food Chemistry

      Volume: 218 Pages: 561-568

    • DOI

      10.1016/j.foodchem.2016.09.102

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp. PCC 68032016

    • Author(s)
      Ishikawa Y, Miyagi A, Haishima Y, Ishikawa T, Nagano M, Yamaguchi M, Hihara Y, Kawai-Yamada M
    • Journal Title

      Journal of Plant Physiology

      Volume: 205 Pages: 105-112

    • DOI

      10.1016/j.jplph.2016.09.002

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] イネ品種間差を利用したメタボローム解析によるシュウ酸蓄積機構の解明2019

    • Author(s)
      宮城敦子、安達俊輔、大川泰一郎、川合真紀
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メタボローム解析から探るイネのシュウ酸代謝機構2018

    • Author(s)
      宮城敦子
    • Organizer
      第12回メタボロームシンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] イネにおけるイソクエン酸リアーゼ(ICL)における生理機能解析2018

    • Author(s)
      宮城敦子、西丸拓也、尾﨑莉沙子、石川寿樹、山口雅利、川合真紀
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Screening of low oxalate rice plants from ion beam-irradiated seeds2018

    • Author(s)
      Atsuko Miyagi, Takuya Saimaru, Nozomi Harigai, Toshiki Ishikawa, Masatoshi Yamaguchi, Maki Kawai-Yamada
    • Organizer
      16th International Symposium on Rice Functional Genomics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネにおける染色体部分置換がシュウ酸蓄積に及ぼす影響2018

    • Author(s)
      宮城敦子、安達俊輔、野口航、常田岳志、臼井靖浩、中村浩史、酒井英光、長谷川利拡、山本敏夫、大川泰一郎、川合真紀
    • Organizer
      第36回日本植物細胞分子生物学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Impact of ion beam-irradiation on metabolisms in oxalate rich plant2018

    • Author(s)
      Atsuko Miyagi, Sayaka Kitano, Yutaka Oono, Yoshihiro Hase, Issay Narumi, Masatoshi Yamaguchi, Maki Kawai-Yamada
    • Organizer
      Plant Biology 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シュウ酸蓄積機構におけるイネ品種間の代謝比較解析2018

    • Author(s)
      宮城敦子, 安達俊輔, 野口航, 常田岳志, 臼井靖浩, 中村浩史, 酒井英光, 長谷川利拡, 山本敏央、大川泰一郎, 川合真紀
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] イネにおけるシュウ酸蓄積機構の解析2017

    • Author(s)
      宮城敦子、西丸拓也、針谷のぞみ、尾﨑莉沙子、大野豊、長谷純宏、長野稔、石川寿樹、山口雅利、川合真紀
    • Organizer
      日本植物生理学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Impacts of high CO2 and nutrients to the metabolic pathway of oxalate rich plant2017

    • Author(s)
      Atsuko Miyagi, Maki Kawai-Yamada
    • Organizer
      Metabolomics 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] イオンビーム育種による低シュウ酸イネの作出2017

    • Author(s)
      宮城敦子, 西丸拓也, 針谷のぞみ, 大野豊, 長谷純宏, 長野稔, 石川寿樹, 山口雅利, 川合真紀
    • Organizer
      日本植物細胞分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] イネにおけるICLの機能解析2017

    • Author(s)
      宮城敦子, 西丸拓也, 針谷のぞみ, 大野豊, 長谷純宏, 長野稔, 石川寿樹, 山口雅利, 川合真紀
    • Organizer
      日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 宮城敦子, 安達俊輔, 野口航, 常田岳志, 臼井靖浩, 中村浩史, 酒井英光, 長谷川利拡, 山本敏央、大川泰一郎, 川合真紀2017

    • Author(s)
      イネ品種間差を利用したシュウ酸蓄積機構の解明
    • Organizer
      メタボロームシンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 根圏のAlが高シュウ酸植物に及ぼす影響2016

    • Author(s)
      宮城敦子、橋田慎之介、川合真紀
    • Organizer
      日本植物学会
    • Place of Presentation
      那覇
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] イオンビーム照射イネ系統におけるメタボローム解析2016

    • Author(s)
      宮城敦子、西丸拓也、大野豊、長谷純宏、川合真紀
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      上田
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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