| Project/Area Number |
16K21008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
Patmawati Lili Jia
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ポリマー / 蛋白質 / 複合化 / 自己集合 / セルロソーム / 検出プローブ / タンパク質 / タンパク質ポリマー / 重合 / 集積 / 機能性材料 |
|---|
| Outline of Final Research Achievements |
Conjugating functional proteins into a large assembly often results in an enhancement of overall functionalities.Here, a SpyCatcher polymer is prepared through catalytic reaction of horseradish peroxidase (HRP) and serves as a polymeric proteinaceous scaffold for construction of protein assemblies. Taking advantage of the favorable SpyCatcher and SpyTag interaction, SpyTagged proteins can
be easily assembled onto the polymeric SpyCatcher scaffold with controllable binding ratio and site specificity. We demonstrated construction of ratio-controllable binary artificial hemicellulosomes by assembling endoxylanase and arabinofuranosidase and biosensing probe by conjugating SpyTagged Nanoluc and protein G onto SpyCatcher polymer. Through this studies, we managed to obtain knowledge of condition of protein polymer preparation and assemblies of proteins onto the protein polymers.
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