Construction of an artificial cell system having cooperation with chemical reaction and dynamics

• Project/Area Number: 16K21034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess; Life / Health / Medical informatics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Morita Masamune 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (90708504)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 人工細胞 / リポソーム / エマルション / DNAナノテクノロジー / 微生物 / 油中水滴 / 人工細胞膜 / DNAナノテク / ドロップレット / 人工細胞システム / DNAゲル / マイクロリアクタ / マイクロナノデバイス / 生物物理

Outline of Final Research Achievements

In this proposal, we aimed to construct and control of the artificial cell-like system having cooperation with chemical reaction and dynamics. Through this attempt, we succeeded in constructing DNA-based micro-sized architectures and living cell-containing artificial cells. These findings and developed methods in this study are expected to be used for creating unique artifacts by combining various biological molecules or cells.

Academic Significance and Societal Importance of the Research Achievements

本研究で構築されたDNAマイクロ構造体は，これまでマイクロスケールでは作製が困難であった幾何学的に複雑な構造を分子の自己組織化反応のみで構成できた点が意義深く，また作製方法も非常にシンプルで今後様々な分子を用いて構造物を作成する基礎技術として重要である。また生細胞を増殖させる人工細胞は，今後，自然界に存在する生物資源の確保，この技術を利用することによる薬剤スクリーニングなどの産業応用が期待される。

Research Products

• [Journal Article] Bacterial Cell Culture at the Single-Cell Level Inside Giant Vesicles (2019)
  • Author(s): Masamune Morita, Yuri Ota, Kaoru Katoh, Naohiro Noda
  • Journal Title: Journal of Visualized Experiments Volume: 146:e59555 Issue: 146 Pages: 1-7
  • DOI: 10.3791/59555
  • Peer Reviewed
• [Journal Article] Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures (2018)
  • Author(s): Masamune Morita, Kaoru Katoh, Naohiro Noda
  • Journal Title: ChemistryOpen Volume: 7 Issue: 11 Pages: 845-849
  • DOI: 10.1002/open.201800126
  • Peer Reviewed / Open Access
• [Journal Article] DNA cytoskeleton for stabilizing artificial cells (2017)
  • Author(s): Kurokawa Chikako、Fujiwara Kei、Morita Masamune、Kawamata Ibuki、Kawagishi Yui、Sakai Atsushi、Murayama Yoshihiro、Nomura Shin-ichiro M.、Murata Satoshi、Takinoue Masahiro、Yanagisawa Miho
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 114 Issue: 28 Pages: 7228-7233
  • DOI: 10.1073/pnas.1702208114
  • Peer Reviewed / Open Access
• [Journal Article] Formation of DNA micro-skeleton structures in water-in-oil microdroplets (2017)
  • Author(s): M. Morita, S. M. Nomura, S. Murata, M. Yanagisawa, M. Takinoue
  • Journal Title: Proc. microTAS Volume: 3C1-4 Pages: 159-160
  • Peer Reviewed
• [Journal Article] Self-Assembled Microcapsule of Amphiphilic Janus DNA Nanoplates at the Water-Oil Interface (2016)
  • Author(s): Daisuke Ishikawa, Yuki Suzuki, Chikako Kurokawa, Masayuki Ohara, Masamune Morita, Miho Yanagisawa, Ryuji Kawano, Masayuki Endo, Masahiro Takinoue
  • Journal Title: Proc. microTAS Volume: - Pages: 116-117
  • Peer Reviewed
• [Presentation] 細胞内小器官型人工細胞を指向したジャイアントベシクル内微生物培養法の構築 (2019)
  • Author(s): 森田雅宗，加藤薫，野田尚宏
  • Organizer: 第２回分子ロボティクス年次大会
• [Presentation] Evaluation of membrane shape deformation of giant vesicles prepared by droplet transfer method (2018)
  • Author(s): Masamune Morita, Naohiro Noda
  • Organizer: 日本生物物理学会第56回年会
• [Presentation] 細胞サイズリポソーム(人工細胞)内での微生物培養 (2018)
  • Author(s): 森田雅宗，加藤薫，野田尚宏
  • Organizer: 「細胞を創る」研究会11.0
• [Presentation] Formation of DNA micro-skeleton structures in water-in-oil microdroplets (2017)
  • Author(s): M. Morita, S. M. Nomura, S. Murata, M. Yanagisawa, M. Takinoue
  • Organizer: microTAS2017
  • Int'l Joint Research
• [Presentation] Generation of complex self-assembled DNA microstructures inside water-in-oil microdroplets (2017)
  • Author(s): M. Morita, S. M. Nomura, S. Murata, M. Yanagisawa, M. Takinoue
  • Organizer: 19th IUPAB congress and 11th EBSA congress
  • Int'l Joint Research
• [Presentation] マイクロ液滴界面を利用したRNA転写可能なDNAマイクロ構造体の構築 (2017)
  • Author(s): 渡邉理佐 , 森田雅宗, 柳澤実穂 , 瀧ノ上正浩
  • Organizer: 「細胞を創る」研究会 10.0
• [Presentation] Construction of artificial cell nuclei with RNA transcription capability using a microdroplet interface (2017)
  • Author(s): R. Watanabe, M. Morita, M. Yanagisawa, M. Takinoue
  • Organizer: 第55 回日本生物物理学会
• [Presentation] Formation of self-assembled DNA microarchitectures on a cationic lipid membrane surface (2016)
  • Author(s): Masamune Morita, Shin-ichiro M. Nomura, Satoshi Murata, Miho Yanagisawa, Masahiro Takinoue
  • Organizer: The 54th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Construction of DNA micro-structures with RNA transcription sequences using the interface of microdroplets (2016)
  • Author(s): Risa Watanabe, Masamune Morita, Miho Yanagisawa, Masahiro Takinoue
  • Organizer: The 54th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] 荷電脂質膜面上での自己組織化DNA マイクロ構造体の構築 (2016)
  • Author(s): 森田 雅宗, 野村 M. 慎一郎, 村田 智, 柳澤 実穂, 瀧ノ上 正浩
  • Organizer: 「細胞を創る」研究会9.0
  • Place of Presentation: 早稲田大学国際会議場井深大記念ホール
  • Year and Date: 2016-11-21
• [Presentation] マイクロ液滴界面を利用したRNA転写配列を 有するDNAフラクタルマイクロ構造体の構築 (2016)
  • Author(s): 渡邉理佐, 森田雅宗, 瀧ノ上正浩
  • Organizer: 「細胞を創る」研究会9.0
  • Place of Presentation: 早稲田大学国際会議場井深大記念ホール
  • Year and Date: 2016-11-21
• [Presentation] 細胞サイズ油中水滴界面における自己組織化DNAマイクロ構造の形成 (2016)
  • Author(s): 森田 雅宗
  • Organizer: 非線形ソフトマター研究会2016
  • Place of Presentation: 横浜市民ふれあいの里 上郷・森の家
  • Year and Date: 2016-10-29
• [Presentation] Construction of self-assembled DNA microstructures for synthesis of molecular robots (2016)
  • Author(s): Masamune Morita, Shin-ichiro M. Nomura, Satoshi Murata, Miho Yanagisawa, Masahiro Takinoue
  • Organizer: Chem-Bio Informatics Society (CBI) Annual Meeting 2016
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2016-10-25
• [Presentation] Self-Assembled Microcapsule of Amphiphilic Janus DNA Nanoplates at the Water-Oil Interface (2016)
  • Author(s): Daisuke Ishikawa, Yuki Suzuki, Chikako Kurokawa, Masayuki Ohara, Masamune Morita, Miho Yanagisawa, Ryuji Kawano, Masayuki Endo, Masahiro Takinoue
  • Organizer: The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS2016)
  • Place of Presentation: Convention Center Dublin, Ireland
  • Year and Date: 2016-10-09
  • Int'l Joint Research
• [Presentation] 荷電脂質膜面上で自己組織化されたDNA マイクロ構造体の構築 (2016)
  • Author(s): 森田 雅宗, 野村 M. 慎一郎, 村田 智, 柳澤 実穂, 瀧ノ上 正浩
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2016-09-07
• [Presentation] Self-assembly of DNA microstructures on a cationic lipid interface of water-in-oil microdroplet (2016)
  • Author(s): Masamune Morita, Shin-ichiro M. Nomura, Satoshi Murata, Miho Yanagisawa, Masahiro Takinoue
  • Organizer: The 22nd International Conference on DNA Computing and Molecular Programming (DNA22)
  • Place of Presentation: Ludwig-Maximilians-Universitat (LMU), Germany
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] 荷電脂質膜界面におけるDNA ミクロ構造の形成 (2016)
  • Author(s): 森田 雅宗, 石川大輔，野村 M. 慎一郎, 村田 智, 柳澤 実穂, 瀧ノ上 正浩
  • Organizer: 化学とマイクロ・ナノシステム学会（CHEMINAS33rd）
  • Place of Presentation: 東京大学生産技術研究所
  • Year and Date: 2016-04-25
• [Patent(Industrial Property Rights)] 固定具及びその使用 (2016)
  • Inventor(s): 五味清佳, 菅ヶ谷暢康, 大森衣里子, 瀧ノ上正浩, 森田雅宗
  • Industrial Property Rights Holder: 五味清佳, 菅ヶ谷暢康, 大森衣里子, 瀧ノ上正浩, 森田雅宗
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-231378
  • Filing Date: 2016-11-29