Identification of a novel anti-mycobacterial agent in human serum

• Project/Area Number: 16K21044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology); Infectious disease medicine
• Research Institution: Osaka City University (2017); Niigata University (2016)
• Principal Investigator: Yamaguchi Takehiro 大阪市立大学, 大学院医学研究科, 助教 (40726080)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 高齢者結核 / 血清マーカー / 感染症 / 潜在性結核

Outline of Final Research Achievements

In the presence of human serum, Mycobacterium tuberculsis and BCG cannot grow. In this study, we try to explore a novel anti-mycobacterial agent in human serum, which may suppress mycobacterial growth in healthy persons. We first expected that the exosomes in serum might be involved in this phenomenon, but serum exosomes did not have anti-mycobacterial effect. Rather, exosome-depleted serum could suppress mycobacterial growth. Anti-mycobacterial activity of exosome-depleted serum may be brought by anti-bacterial peptide, such as cathelicidin or defensin, and further examination is required.

Research Products

• [Journal Article] Capillary Degeneration and Right Ventricular Remodeling Due to Hypoxic Stress with Sugen5416 (2017)
  • Author(s): Woo Eiki、Kato Ryuji、Imano Hideki、Fujiwara Yuji、Ijiri Yoshio、Okada Yoshikatsu、Yamaguchi Takehiro、Izumi Yasukatsu、Yoshiyama Minoru、Katsumata Takahiro、Hayashi Tetsuya
  • Journal Title: Current Vascular Pharmacology Volume: 15 Issue: 6 Pages: 589-598
  • DOI: 10.2174/1570161115666170427154217
  • Peer Reviewed
• [Journal Article] Mycobacterial DNA-binding protein 1 is critical for long term survival of Mycobacterium smegmatis and simultaneously coordinates cellular functions. (2017)
  • Author(s): Enany S, Yoshida Y, Tateishi Y, Ozeki Y, Nishiyama A, Savitskaya A, Yamaguchi T, Ohara Y, Yamamoto T, Ato M, Matsumoto S.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 6810-6810
  • DOI: 10.1038/s41598-017-06480-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells. (2017)
  • Author(s): Kitajima S, Lee KL, Hikasa H, Sun W, Huang RY, Yang H, Matsunaga S, Yamaguchi T, Araki M, Kato H, Poellinger L
  • Journal Title: Oncotarget. Volume: 8 Issue: 70 Pages: 114481-114494
  • DOI: 10.18632/oncotarget.23010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Macrophage-derived exosomes induce inflammatory factors in endothelial cells under hypertensive conditions. (2017)
  • Author(s): Osada-Oka M, Shiota M, Izumi Y, Nishiyama M, Tanaka M, Yamaguchi T, Sakurai E, Miura K, Iwao H.
  • Journal Title: Hypertens Res Volume: 40 Issue: 4 Pages: 353-360
  • DOI: 10.1038/hr.2016.163
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 抗酸菌の長期の生存に必須な細胞機能のヒストン様タ ンパク質依存的な制御 (2017)
  • Author(s): 西山 晃史, Shymaa Enany, 立石 善隆, 尾関 百合子, Anna G. Savitskaya, 山口 雄大, 西田 由貴子, 阿戸 学, 松本 壮吉
  • Organizer: 日本細菌学会
  • Place of Presentation: 仙台国際センター展示棟（宮城県仙台市）
  • Year and Date: 2017-03-19
• [Presentation] 遅延型過敏反応から検討した MDP1 および G9.1 からなる結核ブースターワクチン候補の免疫条件 (2017)
  • Author(s): 前山 順一, 山崎 利雄, 林 大介, 山本 十糸子, 尾関 百合子, 鈴木 史子, 山口 雄大, 松本 壮吉, 伊保 澄子, 山本 三郎
  • Organizer: 日本細菌学会
  • Place of Presentation: 仙台国際センター展示棟（宮城県仙台市）
  • Year and Date: 2017-03-19
• [Presentation] RIVAROXABAN ATTENUATES CARDIAC REMODELING DUE TO INTERMITTENT HYPOXIA BY SUPPRESSING THE SYNERGISTIC EFFECTS OF PAR-1 AND PAR-2 (2017)
  • Author(s): Rintaro Mitsuishi, Hideki Imano, Ryuji Kato, Yoshio Ijiri, Takehiro Yamaguchi, Minoru Yoshiyama, Tetsuya Hayashi
  • Organizer: American College of Cardiology 2017, 66th Annual Scietific Session & Expo
  • Place of Presentation: ワシントンD.C.（アメリカ）
  • Year and Date: 2017-03-17
  • Int'l Joint Research
• [Presentation] 遠隔臓器の一過性虚血コンディショニングは心筋梗塞慢性期の心リモデリングを抑制する (2017)
  • Author(s): 山口雄大
  • Organizer: 日本循環薬理学会
• [Presentation] 心血管リモデリングにエキソソームが果たす役割 (2017)
  • Author(s): 山口雄大
  • Organizer: 日本循環制御医学会
  • Invited
• [Presentation] Rivaroxaban, but Not PAR-2 Antagonist, Attenuates Cardiac Remodeling Due to Intermittent Hypoxia in Sleep Apnea Model Mice (2016)
  • Author(s): Saki Morita, Ayumi Fujiwara, Toshiki Kondo, Kenji Fujii, Hideki Imano, Ryuji Kato, Yoshio Ijiri, Takehiro Yamaguchi, Yasukatsu Izumi, Minoru Yoshiyama, Tetsuya Hayashi
  • Organizer: American Heart Association Scientific Sessions 2016
  • Place of Presentation: ニューオリンズ（アメリカ）
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Rivaroxaban Attenuates Diastolic Dysfunction and Cardiovascular Remodeling Induced by Intermittent Hypoxia in Mice (2016)
  • Author(s): Toshiki Kondo, Ayumi Fujiwara, Kaoru Shimada, Kenji Fujii, Hideki Imano, Ryuji Kato, Yoshio Ijiri, Takehiro Yamaguchi, Minoru Yoshiyama, Tetsuya Hayashi
  • Organizer: 日本心不全学会
  • Place of Presentation: ロイトン札幌・ホテルさっぽろ芸文館（北海道札幌市）
  • Year and Date: 2016-10-07
• [Presentation] Macrophage-derived exosomes under hypertensive conditions cause inflammation in endothelial cells (2016)
  • Author(s): Yasukatsu Izumi, Takehiro Yamaguchi, Mayuko Osada-Oka, Masayuki Shiota, M Tanaka, Katsuyuki Miura, Shuhei Tomita, Hiroshi Iwao
  • Organizer: European Society of Cardiology Congress 2016
  • Place of Presentation: ローマ（イタリア）
  • Year and Date: 2016-08-27
  • Int'l Joint Research