Challenges for synthesis of custom-made artificial enzymes based on chemical synthesis of protein
Project/Area Number |
16K21077
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
Chemical pharmacy
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Research Institution | Shizuoka University |
Principal Investigator |
Sato Kohei 静岡大学, 工学部, 助教 (30756705)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | ペプチド / タンパク質 / チオエステル / ヒドラジド / チアゾリジン / ペプチドチオエステル / タンパク質化学合成 / ペプチド固相合成 / ペプチドヒドラジド |
Outline of Final Research Achievements |
In this study, we successfully developed a method that can directly synthesize N-terminal thiazolidine-containing peptide thioester, which is an essential synthetic intermediate for complete chemical synthesis of proteins, from the corresponding hydrazide. This type of transformation has not been achieved by conventional methods. We also developed a novel solid-phase chemical ligation method for efficient protein synthesis, with peptide hydrazide as the key molecule. These results will find great utility in the preparation of useful artificial proteins.
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Academic Significance and Societal Importance of the Research Achievements |
生体高分子のひとつであるタンパク質は様々な活性を示し、食品・医薬品分野から工業分野まで広く産業応用されている。これら有用タンパク質を自在にデザインしナノレベルでファインチューニングされた人工タンパク質を創出可能な技術として、化学合成法があげられる。本研究で得られた成果は、化学合成法によりタンパク質を合成する際の技術的課題を解決する画期的なものであり、タンパク質を対象とする多様な分野への波及効果も大きい。
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] The role of chemisorption for push-pull chromophores on SiO2surfaces in non-electrically poling host-guest NLO polymers2017
Author(s)
A. Sugita, K. Ito, Y. Sato, R. Suzuki, K. Sato, T. Narumi, N. Mase, Y. Takano, T. Matsushita, S. Tasaka, Y. Kawata
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Journal Title
J. Photochem. Photobiol., A
Volume: 340
Pages: 35-45
DOI
Related Report
Peer Reviewed
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[Journal Article] Labelling of endogenous target protein via N-S acyl transfer-mediated activation of N-sulfanylethylanilide2016
Author(s)
M. Denda, T. Morisaki, T. Kohiki, J. Yamamoto, K. Sato, I. Sagawa, T. Inokuma, Y. Sato, A. Yamauchi, A. Shigenaga, A. Otaka
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Journal Title
Org. Biomol. Chem.
Volume: 14
Pages: 6244-6251
DOI
Related Report
Peer Reviewed
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[Presentation] イメージング質量分析法における 超臨界洗浄法の開発2017
Author(s)
松下祥子, 正木紀隆, 佐藤浩平, 早坂孝宏, Thanai PAXTON, 杉山栄二, 佐藤太, 寺崎真樹, 惠淑萍, 千葉仁志, 間瀬暢之, 瀬藤光利
Organizer
日本薬学会第137年会
Place of Presentation
仙台国際センター他(宮城県仙台市)
Year and Date
2017-03-24 – 2017-03-27
Related Report
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[Presentation] Improvement of peptides imaging on tissue by supercritical fluid wash of lipids for matrix-assisted laser desorption/ionization mass spectrometry2016
Author(s)
Shoko Matsushita, Noritaka Masaki, Kohei Sato, Takahiro Hayasaka, Eiji Sugiyama, Shu-Ping Hui, Hitoshi Chiba, Nobuyuki Mase, Mitsutoshi Setou
Organizer
64th Conference on mass spectrometry and allied topcs
Place of Presentation
Henry B. Gonzalez Convention Center(San Antonio, Texas, USA)
Year and Date
2016-06-05 – 2016-06-09
Related Report
Int'l Joint Research
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