| Project/Area Number |
16K21091
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
Biomolecular chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | RNA / DNA / ケミカルライゲーション / 核酸医薬 / 核酸連結反応 / ホスホロチオエート / 核酸 / ライゲーション / 化学合成 / 有機化学 / ｍRNA / 化学合成法 / 薬学 |
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| Outline of Final Research Achievements |
We developed chemical ligation reaction for oligonucleotides (ONs) that enables the synthesis of long ONs such as mRNA etc. In the ON synthesis for ON medicines, chemical synthesis is optimal because high molecular homogeneity and tolerance for unnatural structure are required, but the current solid phase synthesis has an upper limit on the length of the products. Therefore, it is necessary to couple the fragments obtained by the solid phase synthesis by chemical reaction. We developed new chemical ligation reactions for ONs, which afford ligated strands having a linkage structure similar to that of natural ONs.
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