| Project/Area Number |
16K21101
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
General physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タイトジャンクション / 浸透圧 / アクチン / クローディン / 静水圧 / 癌 / 細胞極性 / 細胞増殖 / プロテインキナーゼA |
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| Outline of Final Research Achievements |
In this study, I attempted to elucidate the mechanism of the regulation of epithelial functions by the extracellular environment.
In MDCK cells, I revealed that claudin-2, transmembrane proteins in tight junctions, act as the sensor of the osmotic gradient between apical and basal sides to regulate several cell functions including transepithelial transport and cytoskeleton. Furthermore, I succeeded in establishing claudin-2 and claudin-4 double knockout cells and revealed the physiological role of claudin-4.
This study elucidates the mechanism in the epithelial cells to sense extracellular environment and regulate cell functions. This study also contributes to set a foundation for the clinical application for the cancer therapy in the future.
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