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Characteristics and behavior of refractory dissolved organic matter produced by bacteria in Lake Biwa

Research Project

Project/Area Number 16K21105
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Environmental dynamic analysis
Research InstitutionKyoto University

Principal Investigator

Kusakabe Taketoshi  京都大学, 工学研究科, 助教 (40462585)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords琵琶湖 / 溶存有機物 / 難分解性有機物 / 微生物群集 / 精密質量分析 / 生分解性試験
Outline of Final Research Achievements

The refractory (non-biodegradable) organic matter has been increasing in lakes, and it is of crucial importance to understand the origin, characteristics, and behavior of natural organic matter. We focused on the refractory dissolved organic matter (R-DOM) produced by microbial communities, and the objective of this research is to demonstrate its chemical characteristics and behavior in the Lake Biwa watershed. The Orbitrap mass spectrometry with electrospray ionization (ESI) was applied to DOM in Lake Biwa and inflowing rivers, and DOM produced by microorganisms and bacterial strains from several organic substrates, e.g., glucose, glutamate, Microcystis aeruginosa. As a result, the role and function of microbial community in Lake Biwa are significant as production of Lake Biwa DOM. And, it was suggested that the contribution of allochthonous DOM is not small.

Academic Significance and Societal Importance of the Research Achievements

本研究では、微生物群集が介在する「微生物炭素ポンプ(microbial carbon pump)」が長期的な炭素貯留プロセスとして駆動していることに鑑みて、微生物食物網(microbial food web)の結節点として細菌が重要な鍵になると考え、微生物群集が産出する有機物に注目した。本研究で明らかとなった難分解性有機物の化学的描像や環境動態に関する知見は、湖沼における物質循環や水質形成メカニズムに対する理解の深化のみならず、健全な湖沼生態系の創出、持続可能な水利用、等々に資するものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) (of which Int'l Joint Research: 3 results) Remarks (1 results)

  • [Journal Article] Evaluation of algal photosynthesis inhibition activity for dissolved organic matter with the consideration of inorganic and coloring constituents2019

    • Author(s)
      Misaki Kentaro, Morita Yuhei, Kobayashi Kentaro, Sugawara Yuichi, Shimizu Yoshihisa, Kusakabe Taketoshi
    • Journal Title

      Chemosphere

      Volume: 224 Pages: 333-342

    • DOI

      10.1016/j.chemosphere.2019.02.106

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 琵琶湖北湖の底質から溶出する有機物量の推定およびその特性解析2018

    • Author(s)
      沈尚, 日下部武敏, 橋本宗樹, 清水芳久
    • Journal Title

      環境システム計測制御学会誌

      Volume: 23 Pages: 164-171

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] DNA変性処理を必要としない水環境中の細菌増殖解析法の検討2019

    • Author(s)
      吉野魁人, 日下部武敏, 沈尚, 清水芳久
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 琵琶湖の物質循環におけるウイルス分流の重要性2019

    • Author(s)
      沈尚, 日下部武敏, 清水芳久
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Viral hot spot in Lake Biwa, Japan2018

    • Author(s)
      Shang Shen, Taketoshi Kusakabe, Yoshihisa Shimizu
    • Organizer
      The 17th World Lake Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 琵琶湖北湖における細菌の死亡要因にしめるウイルスの影響2017

    • Author(s)
      沈 尚、日下部 武敏、橋本 宗樹、山内 渚、清水 芳久
    • Organizer
      京都大学環境衛生工学研究会第39回シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 熱化学分解GC/MS法による藻類の生分解特性の評価2017

    • Author(s)
      日下部武敏, 橋本宗樹, 山内渚, 沈尚, 清水芳久
    • Organizer
      日本腐植物質学会第32回講演会
    • Place of Presentation
      名城大学(愛知県名古屋市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Molecular Characteristics and Diversity of Dissolved Organic Matter Produced by an Alphaproteobacteria, Novosphingobium lentum from Glucose and Glutamate2016

    • Author(s)
      Sheg S., Yamagata S., Hashimoto H., Kusakabe T., Shimizu Y.
    • Organizer
      The 18th International Conference of International Humic Substances Society
    • Place of Presentation
      Kanazawa Bunka Hall (Kanazawa, Ishikawa)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microbial Transformation and Dissolution Processes of Fine Particulate Organic Matter (FPOM) in Lake Biwa2016

    • Author(s)
      Hashimoto H., Yamagata S., Shin S., Kusakabe T., Shimizu Y., Katahira M.
    • Organizer
      The 7th International Symposium of Advanced Energy Science
    • Place of Presentation
      Kyoto University (Kyoto and Uji)
    • Year and Date
      2016-09-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 研究内容紹介ページ

    • URL

      http://www.eqc.kyoto-u.ac.jp/management/topics/nom/research.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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