Role of a novel B-1 cell subset in anti-inflammatory processes

• Project/Area Number: 16K21107
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry; Biological pharmacy
• Research Institution: Jichi Medical University
• Principal Investigator: Watanabe Sachiko 自治医科大学, 医学部, 助教 (80770619)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 免疫 / B細胞 / B-1細胞 / IL-10 / 免疫学

Outline of Final Research Achievements

SPA-1 is a specific negative regulator of Rap1 that mediates broad cellular activities. Because our preliminary results suggest that SPA-1 knockout mice show a lack of a novel B-1 cell subset that acts as anti-inflammatory properties, we investigated the role of the cell subset in anti-inflammatory processes. The new B-1 cell subset isolated from WT cells produced a large amount of an anti-inflammatory cytokine IL-10. In this cell subset, the expression levels of integrin and PD-L2 were higher whereas B cell differentiation-related transcription factors tended to be lower than other cell subsets. Furthermore, this cell subset was not present in young WT mice. These findings suggest that SPA-1 regulates the differentiation of novel B-1 cell subset which has anti-inflammatory function.