| Project/Area Number |
16K21116
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
Konagaya Shuhei 京都大学, ウイルス・再生医科学研究所, 特定研究員 (60770295)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 膵島 / 細胞外電位 / 多能性幹細胞 / 分化誘導 / iPS細胞 / 細胞外電位測定 / インスリン / Microelectrode array / 移植・再生医療 / 糖尿病 / 生物・生体工学 |
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| Outline of Final Research Achievements |
To develop a new method of functional evaluation of pluripotent stem cell-derived islet, we have recorded the electrical activity of the cells using planar, extracellular electrodes arranged in a microelectrode array (MEA). Although isolated-mouse islet cells poorly adhered on MEA, the cells adhered well on the laminin-332-coated MEA. We succeeded in recording of the electrical activity of islet cells in response to glucose stimulation. We also recorded the activity of human iPS cell-derived islet cells. However, the electrical signal was very low and noisy. It might be caused by insufficient maturation and heterogeneity of the differentiated cells. As a solution, we newly developed a culture method for expansion and purification of pancreatic cells derived from human iPS cells.
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